FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30926268
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT538990.17428226386707893No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA490650.15865153855615557No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG424190.13716171637651203No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG371820.12022789170681701No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA339550.10979339634513935No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT339390.10974166039044866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT54900.035.9059946
AGACCGC66700.029.8386654
CCGCGTT71200.027.9527957
TCGTATG70600.027.61847342-43
CGCGTTC76400.026.1124218
CGTACGG23450.025.7248063
GCGTTCT82250.024.1974379
GCGTACG26350.023.7949352
GACCGCG87900.022.5880435
ATGCCGT89550.022.1983746-47
CAGACCG92300.022.1288053
TCTCGTA92750.019.8705140-41
CTCGCTA136950.019.769886
TATGCCG63000.018.84910246-47
TCGCTAT144650.018.7174917
GTATGCC111150.017.92725444-45
CGCTATG156050.017.3196758
GCCGTCT116600.016.96710248-49
GTCGGTT43800.016.9233511
GTACGGA37650.016.9056194
Produced by FastQC (version 0.11.5)