FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30545608
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG600820.19669603564610663No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG500670.16390899798098632No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG425690.139362097490415No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT404960.13257552444200818No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA321600.1052851853529974No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT314740.10303936330224626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT44900.035.2280966
CCGCGTT55350.028.1479997
AGACCGC59350.026.651084
CAGACCG68650.023.5250023
GCGTTCT68500.023.0911169
CGATTAG43950.021.8315094
GTTCGAT45600.021.5686341
CGCGTTC75000.021.153228
GACCGCG77950.020.6573625
TCGATTA46250.019.8215143
GTCGGTT41650.017.9102551
TCGCTAT112150.017.788587
CTCGCTA113450.017.5847456
TTCGATT58900.016.7741032
CGCTATG120750.016.5609878
CGGTTCG34900.016.4683933
GAACGAT51650.015.8179244-45
AATACGG58800.015.26777532-33
AACGATC58750.015.24034446-47
CAATACG54350.014.9884230-31
Produced by FastQC (version 0.11.5)