FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30545608
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGC591280.1935728370507472No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG561470.1838136598885182No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCG544450.17824166407164002No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA426710.13969602438425843No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT424860.13909037266503255No Hit
CCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCG398160.1303493451497184No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG380390.124531814852073No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT374540.12261664590208843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAC177700.024.0058231
TGGCGCG184700.022.6319963
GGCGCGT199550.021.3047084
ACGTGCG217850.020.2346026
CCGACCG193450.020.0121237
TTGAACG56250.019.9293777
GGCGATC48450.019.706982
AACACGT226550.019.4576783
TTACAGC303750.018.8603082
GTTACAG323900.018.0687241
GGTCGAC49400.017.9821821
TCTAACA246450.017.8484272
TGCGCGA253850.017.6454359
TACAGCC332650.017.3498553
CCCCGAC226000.017.297995
TAACACG256750.017.1324042
GCGCGTG255600.016.8930195
CGCGTGC253900.016.7629156
ACACGTG269100.016.5927524
CGTGCGC287050.015.5055187
Produced by FastQC (version 0.11.5)