FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30798695
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1368860.4444538965043811No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG664710.2158240795592151No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC568900.1847156186325427No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA522210.1695558854035861No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT499460.16216920879277516No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA422130.13706100209765382No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG389560.12648587870362688No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC372200.120849276243685No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA372070.1208070666630518No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT344230.11176772262590996No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT314380.10207575353436242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT88600.042.245316
CGCGTTC100650.037.140438
AGACCGC102950.036.541374
CCGCGTT102500.036.516437
GACCGCG110000.034.069865
GCGTTCT117050.032.1801269
CAGACCG126850.030.4803473
CGTACGG18400.029.686983
GAGTCTT563700.024.5795764
TGGAGTC578350.024.039092
GGAGTCT588250.023.8686853
AGTCTTG595200.023.685745
GTCTTGG612800.023.2070036
CTTGGAA652600.021.5733268
TTGGAAG657150.021.3155369
TCTTGGA675250.021.173277
GCGTACG26300.020.9502032
AATACGG112350.019.49009132-33
TACGGAG113900.019.03719934-35
AACGATC119900.018.10435346-47
Produced by FastQC (version 0.11.5)