FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30798695
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA455700.14796081457347462No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT419230.13611940376045154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT95700.043.7304041
GGCGATC100900.040.581352
CGATCTG161700.025.7329124
GCGATCT167250.025.1920573
CGGTGGC232450.024.7272221
GGCGCGT253550.022.7618484
TGGCGCG255750.022.5294863
GGTGGCG277800.021.6139621
GCGCGTG290850.019.9407165
CGCGTGC302750.019.1255426
GGTCGAC66700.019.0865961
TGCGACA239450.018.7659456
GCGACAT245950.018.501757
GCGTGCC331500.017.452517
GTGGCGC343450.017.3714662
GCTGCGA270000.016.695424
CTGCGAC271600.016.6320155
CGACATC290050.015.7215248
CGTGCCT393450.014.9943738
GTCGACC87250.014.9174812
Produced by FastQC (version 0.11.5)