FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28971088
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1279820.4417576585318439No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1067760.368560545603258No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT577810.19944366604388486No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT523500.18069739044664113No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC519910.17945822400594688No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT405670.14002580779845067No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA351550.12134511482620189No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG335750.11589140180030517No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA323780.11175969642562267No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC319620.11032378210994354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGTC446150.028.0426252
GAGTCTT448050.027.8388984
GGAGTCT461650.027.1628233
AGTCTTG470900.026.9116885
CGTACGG10650.026.3139613
CTCGCTA61850.025.4300231
GTCTTGG502800.025.128716
TCGCTAT61500.025.1010592
TTGGAAG529000.023.8303179
TCTTGGA560600.023.1563637
CTTGGAA558850.023.0503888
ACCGCGT28600.022.2547426
CGCTATG69600.021.6338523
GCGTACG14350.020.5221842
CGCGTTC31300.020.183258
CCTACGT328600.019.52139324-25
TACGTTC332750.019.3564726-27
CTACGTT334450.018.68999726-27
GACTACC346200.018.6325818-19
CCCTACG336850.018.4933524-25
Produced by FastQC (version 0.11.5)