FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28971088
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA364840.12593244685874413No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT298240.102944010939458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT71650.039.451631
GGCGATC71350.039.2156372
TGGCGCG185650.029.9383683
GGCGCGT206950.027.0612644
GGTGGCG216700.026.1983031
GCGATCT110300.025.755083
CGATCTG111500.025.4330924
GTGGCGC235800.024.0344732
TGCGACA164100.023.7358196
GCGACAT169150.023.2517197
GCGCGTG247900.022.8018155
CGGTGGC192700.021.941811
CTGCGAC184600.021.2800585
GCTGCGA189950.020.5056864
CGCGTGC274650.020.3734556
GCGTGCC300750.018.652677
CGACATC230300.017.2221668
CGTGCCT358600.015.6965178
GGCTGCG256150.015.3558263
GCCACGA115050.013.97525518-19
Produced by FastQC (version 0.11.5)