FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31218027
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT366100.1172719851898392No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA350480.1122684659091364No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG339320.10869360834366631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT75950.046.029616
CCGCGTT80650.043.052697
GACCGCG86350.040.7058335
AGACCGC88200.039.5289044
CAGACCG94500.037.6476023
CGCGTTC93050.037.5196048
GCGTTCT107200.032.700089
TCGTATG72350.025.86693644-45
ATGCCGT85450.021.56785648-49
CTCGCTA89750.020.26996
GAACGAT96200.020.02179744-45
CAATACG98800.019.7352930-31
TACGGAG100150.019.65897634-35
AACGATC100550.019.55714846-47
ATACGGA99650.019.54311632-33
TCGCTAT93800.019.2934277
CGATGCG89400.019.1803683
AATACGG103450.019.0777832-33
GCAACGC102300.018.8284180-81
TCTCGTA89550.018.6443442-43
Produced by FastQC (version 0.11.5)