FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31218027
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA73700.025.39343848-49
GCCGTAT74000.025.1942146-47
GGCGCGT126400.021.7220081
TGGCGCG127200.020.8005143
TCGCCGT93350.020.35346844-45
GGCGATC48400.020.119991
GGTCGAC61300.019.915531
GGGCGAT52950.018.929351
GTCGACC82250.018.481331
TCGACCC83700.017.9338652
GGTCGCC108800.017.37585842-43
CGTGCGG91400.017.253888
GTGGTCG111600.017.11026440-41
GGTGGCG163600.017.0150621
ACGTGCG53050.016.5645566
CGCGTGC167250.016.4160143
CCGTGCG97600.016.0117767
CGCCGTA61950.015.98665246-47
CGACCCG104800.015.7733513
GCGCGTG173900.015.6792242
Produced by FastQC (version 0.11.5)