FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31556390
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT512240.162325284989823No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT438300.13889421445228686No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA373050.11821694433361991No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG346960.10994920521643953No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG322660.10224870462052219No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA321880.10200152805818409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT69400.038.125991
TGGCGCG274800.036.179922
GGCGATC83400.031.555071
GTGGCGC353250.030.8083461
GGCGCGT350450.028.4509433
CGGTGGC211800.027.7440051
GCGCGTG377200.026.5084
GGTGGCG266150.025.1305271
CGTATCA70050.024.41095248-49
CGCGTGC412650.024.2190825
GCCGTAT73950.023.09144246-47
GCGTGCC449550.022.421326
TCGCCGT77400.022.18491244-45
CGTGCCT503400.020.2304427
TGCGACA200300.019.2086966
GCGACAT202800.019.0421687
CGATCTG139300.018.8234083
GCGATCT142300.018.7603843
GCGCACG92400.018.4036876
CGCACGC93050.018.0198887
Produced by FastQC (version 0.11.5)