FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31115107
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC2038000.6549873024701474TruSeq Adapter, Index 7 (100% over 50bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1840880.5916354393381967No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1026180.3298012119964749No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC577610.1856365141215809No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC535360.17205790100609328No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC434660.13969420063379503No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT424120.1363067785690083No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT387830.1246436337178593No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT368670.11848585319022044No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA346720.11143140211602037No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT338360.10874460434926352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG327750.039.3071642-43
ATGCCGT343750.037.0425546-47
TATGCCG316800.036.01328744-45
GCCGTCT351800.035.8238748-49
GTATGCC362850.035.511344-45
TCTCGTA327700.035.0618640-41
CGTATGC347000.032.940642-43
CGTCTTC391450.032.03735750-51
TGCCGTC363200.031.0269246-47
CCGTCTT366850.030.42708248-49
CATCTCG381100.030.13687138-39
ATCTCGT337150.030.03868938-39
TGGAGTC640150.028.6671092
GAGTCTT662100.027.7023964
GGAGTCT664850.027.6949083
AGTCTTG693800.026.7513855
GTCTTGG696550.026.6866596
GCTTGAA525800.025.02965258-59
CTTGGAA740550.024.806218
TTGGAAG754500.024.284659
Produced by FastQC (version 0.11.5)