FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005494182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005494182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31115107
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT2269800.7294848769120414Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA453520.14575556497363162No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT342410.11004622288459429No Hit
GGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACG318950.1025064769984561No Hit
TGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAGG317890.10216580646822138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT81450.043.169911
CGTATCA311150.040.52244648-49
GCCGTAT314750.040.0815446-47
TCGCCGT338750.037.25619544-45
CGCCGTA309400.036.39244-45
GGTCGCC360100.035.95126342-43
CCGTATC316750.035.5172146-47
GGCGATC100050.034.8543782
GTGGTCG374250.034.32575640-41
CGGTGGT396850.032.431338-39
TGGTCGC354950.032.37156340-41
GTCGCCG357500.032.18023742-43
CTCGGTG409400.031.53575336-37
TCTCGGT389150.029.61251834-35
ATCTCGG441950.029.5624334-35
TCGGTGG393500.029.2489536-37
GTATCAT387450.029.15270848-49
TGGCGCG191250.027.697993
GTAGATC469950.027.67401530-31
TCATTAA491750.026.35997852-53
Produced by FastQC (version 0.11.5)