FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005498014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005498014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67601290
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT5011770.741371947192132TruSeq Adapter, Index 18 (97% over 40bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA3082170.45593360718412324No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2693410.39842582885622446No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG2314070.342311515061325No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG2298660.34003197276264996No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG2287840.33843141158992673No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA2236580.33084871605260785No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG2203880.3260115302533428No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2179590.32241840355413337No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2105460.31145263648075355No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1838560.27197114137910683No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1826210.27014425316439966No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1657540.24519354586280823No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1655430.24488142164151008No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1450790.21460980996072707No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1418550.20984067020022842No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC1285040.1900910470791312No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1268480.18764138968354005No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1227330.1815542277373701No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1226230.18139150894901562No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1149640.1700618434944067No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG1144660.1693251711616746No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT1139350.16853968319243612No Hit
CTGTCATTGAATCCTAGATTACTGAGGAGACTGCCCCTGTGGAGGTCCTT1136030.16804856830394804No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG1128620.16695243537512378No Hit
GGGAAGTATCCTGAAACACAGAATTCACTAAATCATGGTATTTCTTTTAT1123810.16624091049150097No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC1091220.16141999657107134No Hit
GCAAGCTGATTATTTTATTGGTATATTGAAGTTAGAGCATGATCACATTA1069410.15819372677651564No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT1047670.15497781181394615No Hit
GCAAGTATCTAATCAAATGATAATTAGGAAGGGAAGTATCCTGAAACACA1043940.1544260471952532No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG989200.14632856858204926No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC974350.14413186493926372No Hit
CAAGTATCTAATCAAATGATAATTAGGAAGGGAAGTATCCTGAAACACAG958110.14172954391846662No Hit
CTTGAATTTTATTGCTTTTGGAGAGGAATGAATTTCTATTACTCAGAAAC948710.140339037908892No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA943760.1396068033612968No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA942840.13947071128376395No Hit
CCTCGATGTGAATGATGCTTTTCATGGAATTTTCTTTTATACCCATGATG933690.13811718681699714No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA905640.13396785771395783No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT905110.13388945684320522No Hit
CTCGATGTGAATGATGCTTTTCATGGAATTTTCTTTTATACCCATGATGT904940.13386430939409588No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC862600.12760111530416063No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT837770.12392810847248625No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA836620.1237579933755702No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG820240.12133496269080071No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC817090.12086899525142199No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC808680.11962493615136634No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC803690.11888678455692192No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA794440.11751846747303195No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT779740.11534395275593114No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGG773090.11436024371724268No Hit
GGTATTTCTTTTATTCTGCATTGCCTTATGATAATTCTTCAAAAAGCAGT764900.11314872837485794No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC760350.11247566429575531No Hit
GCTGATTATTTTATTGGTATATTGAAGTTAGAGCATGATCACATTATGTT728590.1077775290974477No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG724420.10716067696341297No Hit
GCAAGCTGATTGTTTTATTGGTATACTGAAGTTAGAGCATGATGCCATGT722960.1069447047534152No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT705180.10431457742892183No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA699100.10341518630783526No Hit
CCATGATGTCTCTTTGCATGTGAATCAGCTCCAGTCATGGAAAGCATGAG696740.10306608054372927No Hit
GTAGTGTGATGCGGTATGACAAATGAGAATACACGAGTCCAAAGCGAATT683570.10111789286861242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)