FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39234
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA32508.28363154406892No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA31628.059336289952594No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT24676.287913544374776No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT16704.25651220879849No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT16614.233572921445685No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT16034.085741958505378No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT14563.7110669317428764No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT12233.1171942702757813No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT11883.0279859305704235No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT11132.8368252026303717No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT11102.8291787735127696No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT9162.3347096905745017No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT8992.291379925574757No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT8042.0492430035173577No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGTCGCCTTCGCCACCGGT4671.1902941326400571No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG4391.118927460875771No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT4241.0806953152877605No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT4141.055207218229087No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT4041.0297191211704133No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT3920.9991334047000051No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT3820.9736453076413316No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA3660.9328643523474537No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT3140.800326247642351No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA3050.7773869602895448No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT2760.7034714788193914No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT2670.6805321914665852No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT2590.6601417138196463No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT2570.6550440944079116No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT2560.6524952847020441No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA2550.6499464749961767No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT2360.601519090584697No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT2310.5887750420553601No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA2250.5734821838201559No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT2040.5199571799969415No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT1770.4511393179385227No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1730.4409440791150533No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1720.43839526940918594No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA1680.42820003058571643No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT1650.4205536014681144No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1640.41800479176224703No Hit
TTCGTGCCTGAGCGTCAGTTATGTCCCAGGAGGCTGCCTTCGCCATCGGT1630.4154559820563797No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT1590.40526074323291017No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT1580.4027119335270429No Hit
TTCGCGCCTCACCGTCAGTTGCCGTCCAGTTATCCGCCTTCGCCACTGGT1490.37977264617423667No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT1350.3440893102920936No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT1310.33389407146862415No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1270.32369883264515475No Hit
TTCGTGCTTCAGCGTCAGTTATAGTATGGTATGCTGCCTTCGCAATCGGA1250.31860121323342No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1240.31605240352755265No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT1150.2931131161747464No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT1060.27017382882194013No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT1050.26762501911607284No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT1030.2625273997043381No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT970.24723454146913393No Hit
TTCGAGCCTCAGCGTCAGTAATGCCCCGGTACAATGCCTGCGCGATCGGA960.24468573176326655No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT950.24213692205739917No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT940.23958811235153185No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT920.2344904929397971No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT900.2293928735280624No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT870.22174644441046032No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGACCGCCTTCGCCACTGGT850.2166488249987256No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGTAGGCCGCCTTCGCCACTGGT850.2166488249987256No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT820.20900239588112351No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT810.20645358617525614No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGGT800.20390477646938882No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT780.19880715705765406No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT780.19880715705765406No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT780.19880715705765406No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT780.19880715705765406No Hit
TTCGAGCCTCAACGTCAGTTACAGCTTAGCAGGCTGCCTTCGCAATCGGG770.1962583473517867No Hit
TTCGTGCTTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT750.19116072794005198No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT730.18606310852831728No Hit
TTCGAGCCTCAACGTCAGTTACGGTCCAGCAAGCTGCCTTCGCAATCGGA710.18096548911658256No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGCAGGCTGCCTTCGCCATCGGT710.18096548911658256No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT640.16312382117551102No Hit
TTCGTGCTTCAGCGTCAGTTGCAGACCAGGCGACCGCCTTCGCCACTGGT620.15802620176377632No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATCGGT600.1529285823520416No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGCT600.1529285823520416No Hit
TTCGTGCCTCAACGTCAGATATAGCTTGGTAAGCTGCCTTCGCAATCGGT550.1401845338227048No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT550.1401845338227048No Hit
TTCGCGCCTCAGCGTCAGTCTTGGCCCAGAGAATCGCCTTCGCCACTGGT550.1401845338227048No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT530.13508691441097007No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT510.12998929499923537No Hit
TTCGCGCATCAGCGTCAGTTACTGTCCAGCAAATCGCCTTCGCCACTGGT500.127440485293368No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT480.12234286588163328No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAGTCGCCTTCGCCACTGGT480.12234286588163328No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT460.11724524646989855No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC440.11214762705816383No Hit
TTCGTGTCTCAGCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT420.10705000764642911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)