FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39234
Sequences flagged as poor quality0
Sequence length200
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT32018.158739868481419No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTGCTTAAGTCAGTT30187.6923076923076925No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT18454.702553907325279No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT16424.185145537034205No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT13583.4612835805678746No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG12973.305806188509966No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA12433.1681704643931283No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCTGAA11923.0381811693938934No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA11572.948972829688535No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT9562.436662078809196No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA9312.372941836162512No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGATAGCAAGTCAGCT8762.2327573023398073No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT7411.8886679920477136No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA7401.8861191823418464No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT5821.4834072488148033No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAAGGCAAGTCAGAT4991.2718560432278128No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT4941.2591119946984757No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG4331.1036346026405668No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA4291.0934393638170974No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA3991.0169750726410767No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCTTAAGTCCATC3920.9991334047000051No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT3790.9659988785237295No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT3620.9226691135239842No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG3540.9022786358770454No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT3530.899729826171178No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT3520.8971810164653107No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT3290.8385583932303615No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAATACAAGTTAGTT2880.7340571952897996No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGACGGTAAGTCAGGT2730.6958250497017893No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG2410.6142631391140337No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG2360.601519090584697No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA2290.5836774226436254No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG2220.5658357547025539No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT2050.5225059897028088No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGTTGTTAAGTCAGTT1930.49192027323240045No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA1930.49192027323240045No Hit
TCCGGAATTACTGGGTGTAAAGGGAGTGTAGGCGGGATATCAAGTCAGAA1890.48172503440893105No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGCAGGCAAGTCAGGC1830.46643217617372684No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT1660.4231024111739818No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCTGATAAGTTGGAT1650.4205536014681144No Hit
ATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGTTGAGTAAGACAGAT1600.40780955293877763No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGGA1460.37212621705663457No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCGATTAAGTTAGAT1410.3593821685272977No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT1360.34663811999796096No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGTATCACAAGTCAGAA1350.3440893102920936No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGGGCTTAAGTCAGTT1240.31605240352755265No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT1200.3058571647040832No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT1160.2956619258806138No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGTGATTAAGTCAGCG1140.29056430646887904No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGGTATTAAGTCAGTT1140.29056430646887904No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA1120.2854666870571443No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT1090.27782025793954224No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCCTAAGTCCATC1030.2625273997043381No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGCAGTCAAGTCAGCG1020.25997858999847073No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGCAAGTCAGAA1000.254880970586736No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT1000.254880970586736No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGGTAAGTTTGAA990.25233216088086863No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA990.25233216088086863No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT930.23703930264566447No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA900.2293928735280624No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTAAGCAAGTCAGAA860.21919763470459297No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT820.20900239588112351No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT800.20390477646938882No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA800.20390477646938882No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCCGGCAAGTCAGAT770.1962583473517867No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT760.19370953764591936No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCTTTAAGTCAGTT760.19370953764591936No Hit
TCCGGAATGATTGGGCGTAAAGGGCGTGTAGGCGGCGATTTAAGTCTGGA750.19116072794005198No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAA750.19116072794005198No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT730.18606310852831728No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGAT720.1835142988224499No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGAGACAAGTTGAAT710.18096548911658256No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCGAGGCAAGTCAGGC690.17586786970484786No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT690.17586786970484786No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGGGATTAAGTCAGTT670.17077025029311313No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATAGCAAGTCTGAA670.17077025029311313No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCGCCTAAGCGGAAC650.16567263088137837No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGAGAGACAAGTCAGAT600.1529285823520416No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT600.1529285823520416No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTCAGATAAGTTAGAG570.14528215323443952No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGCGGCCCGTTAAGTCTGGA560.14273334352857214No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCTGGTAAGTCAGAT520.13253810470510272No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGAT510.12998929499923537No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGTGCAAGTCTGAA500.127440485293368No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT480.12234286588163328No Hit
TCCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGTATAATAAGTCAGTG480.12234286588163328No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAA470.11979405617576593No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG450.1146964367640312No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG440.11214762705816383No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTCAGAT430.10959881735229648No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT430.10959881735229648No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTGCTTAAGTCAGGT430.10959881735229648No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGCTCGTCGCGTCCGGT420.10705000764642911No Hit
TCCGGAATGACTGGGCGTAAAGGGCGCGTAGGCGGCTGTGTAAGTCTGGA400.10195238823469441No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGAAGGCAAGTTGGAT400.10195238823469441No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACAGCAAGTCAGAT400.10195238823469441No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT400.10195238823469441No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGAA400.10195238823469441No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)