FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40012
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT597014.920523842847148No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA569614.235729281215637No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT506112.648705388383485No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGG19204.798560431870439No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT18924.728581425572329No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT11572.891632510246926No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT10252.5617314805558333No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT8342.084374687593722No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT7801.9494151754473659No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT6561.6395081475557332No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT6151.5370388883335No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT4781.1946416075177446No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT4581.1446566030190943No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA4521.129661101669499No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT3840.9597120863740878No Hit
TTCGAGCCTCAACGTCAGTGATCGTCCAGTAAGCCGCCTTCGCCACTGGT3690.9222233330001001No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA3670.917224832550235No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT3590.8972308307507748No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT3590.8972308307507748No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT3230.807257822653204No Hit
TTCGTGCCTCAGCGTCCGATATAGATTTGTAAGCTGCCTTCGCAATCGGT3190.7972608217534739No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT3050.7622713186044187No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT3030.7572728181545536No Hit
TTCGTGCTTCAGTGTCAGCATCCAGACCAGACGGCCGCCTTCGCCACCGG2790.6972908127561732No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA2790.6972908127561732No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG2690.672298310506848No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATAGGA2520.6298110566829951No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA2480.619814055783265No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT2390.5973208037588723No Hit
TTCGAGCATCAGCGTCAGTTACAATCCAGTAAGCTGCCTTCGCAATCGGA2380.5948215535339398No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCGCTGGT2260.5648305508347495No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT2050.5123462961111667No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT2030.5073477956613016No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT1970.4923522943117065No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1950.48735379386184147No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT1940.48485454363690894No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT1840.45986204138758374No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT1720.42987103868839344No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1720.42987103868839344No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA1600.39988003598920324No Hit
TTCGTGCCTCAGCGTCAGTGATGGCCCAGCAAGTCGCCTTCGCCACTGGT1220.3049085274417675No Hit
TTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1170.29241227631710487No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT1090.27241827451764467No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1000.24992502249325202No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT970.24242727181845447No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT920.22993102069379187No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT870.21743476956912924No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCTAGTGAGCTGCCTTCGCAATCGGA870.21743476956912924No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT850.2124362691192642No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT830.20743776866939917No Hit
TTCGTGCCTCAGCGTCAATCAATGCCCAGATAGCCGCCTTCGCCTCTGGT760.18994301709487155No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT690.1724482655203439No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT680.16994901529541137No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT630.15745276417074877No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG630.15745276417074877No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT560.13995801259622112No Hit
TTCGAGCCTCAGCGTCATTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT550.1374587623712886No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT540.1349595121463561No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT530.13246026192142357No Hit
TTCGAGCCTCAATGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACCGGT460.11496551034689594No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGT450.11246626012196342No Hit
TTCGTGCCTCAGCGTCCGCTATAGATTTGTAAGCTGCCTTCGCAATCGGT450.11246626012196342No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT440.10996700989703088No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT430.10746775967209836No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT420.10496850944716585No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT410.10246925922223332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)