FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43173
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT1092125.29590253167489No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT679315.734371018923865No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT37828.760104695064044No Hit
TTCGAGCTTCAGCGTCAGTTGCGCCCCAGTGAGCTGCCTTCGCAATCGGA15913.6851736038727907No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT12682.9370208231996853No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT10492.429759340328446No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT9552.21203066731522No Hit
TTCGAGCATGAACGTCAGTTACGGATTAGTGTGCTGCCTTCGCAATCGGA9452.1888680425265794No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT7891.8275310958237787No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT7181.6630764598244272No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACCGGT6631.5356820234869015No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT6611.5310494985291734No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCTTTCGCCACTGGT5911.368911125008686No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT4871.1280198272068191No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT4761.102540939939314No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT4631.0724295277140805No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT4100.949667616334283No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT3850.8917610543626805No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT3760.8709146920529034No Hit
TTCGAGCCTCAACGTCAGTGGCGGCTTAGCAGGCTGCCTTCGCAATCGGG3480.8060593426447086No Hit
TTCGAGCCTCAGCGTCAGTTGCACTCCGGACACATGCCTTCGCGATCGGA3350.7759479304194752No Hit
TTCGAGCTTCAGCGTCAGTTGCGCTCCAGTGAGCTGCCTTCGCAATCGGA2660.6161258193778518No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA2640.6114932944201237No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT2210.5118940078289671No Hit
TTCGAGCATCAGCGTCAGTGGTGCTGCGGCATGCTGCCTTCGCAATCGGA2050.4748338081671415No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1940.4493549208996363No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT1870.43314108354758757No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT1720.39839714636462603No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1710.39608088388576196No Hit
TTCGAGCATGAACGTCAGTTACAGTTTAGCAAGCTGCCTTCGCAATCGGG1650.3821833090125773No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGAGAGCCGCCTTCGCAACTGGT1650.3821833090125773No Hit
TTCGAGCCTCAGCGTCAGTGCTGCCCCGGCACAATGCCTTCGCGATCGGA1530.35438815926620804No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT1500.34743937182961576No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1380.31964422208324644No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT1310.30343038473119777No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT1300.30111412225233364No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT1180.2733189725059644No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTCCGGTAAGCTGCCTTCGCAATCGGA1090.25247261019618744No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1050.24320756028073104No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT1020.23625877284413868No Hit
TTCGCATCTCAGCGTCAGCCACAGTCCAGAAAGGCGCCTTCGCCACCGGT910.21077988557663355No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT900.20846362309776945No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT900.20846362309776945No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT880.20383109814004122No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT850.19688231070344892No Hit
TTCGCCTCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACCGGT830.1922497857457207No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT810.18761726078799248No Hit
TCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTG790.18298473583026428No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT790.18298473583026428No Hit
TTCGAGCATTAACGTCAGTTACGGATTAGTGTGCTGCCTTCGCAATCGGA740.17140342343594375No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTGCGGCAGGCTGCCTTCGCAATCGGA720.16677089847821555No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT710.16445463599935145No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT640.14824079864730272No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT630.14592453616843862No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT610.1412920112107104No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT570.132026961295254No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGGT540.12507817385866166No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA520.12044564890093346No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGACAGCCGCCTTCGCCACTGGT520.12044564890093346No Hit
TTCGAGCCTCAGCGTCAGTTATGCCCCGGCACAATGCCTTCGCGATCGGA500.11581312394320525No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGAAGGCTGCCTTCGCCATCGGT480.11118059898547704No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA460.10654807402774884No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT450.10423181154888472No Hit
TTCGCACCTCAGCGTCAATACCGGTCCAGGTGGCCGCCTTCGCCACTGAT450.10423181154888472No Hit
TTCGTGCATGAGCGTCAGTCGTATCCCAGGGGGCTGCCTTCGCCATTGGT440.10191554907002061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)