FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32631
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT460514.11234715454629No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT31899.772915325917072No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT18415.6418742913180715No Hit
TTCGAGCATGAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGA10283.1503784744568044No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT9552.9266648279243666No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG9192.816340289908369No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA8792.693757469890595No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT8062.4700438233581563No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT6071.860194293769728No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA5951.8234194477643957No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT5351.639545217737734No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA5231.6027703717324018No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT4881.495510404216849No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT4371.3392173086941865No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT4261.3055070331892986No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT4001.225828200177745No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT3941.2074407771750788No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT3471.063405963654194No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT3190.9775979896417517No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGAT3140.9622751371395298No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT3080.9438877141368638No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT3020.9255002911341976No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT2650.8121111826177561No Hit
TTCGAGCTTCAGCGTCAGTTGCGCTCCAGTGAGCTGCCTTCGCAATCGGA2580.7906591891146456No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGCAAGCTGCCTTCGCAATCGGA2440.7477552021084245No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT2420.7416260611075358No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT2420.7416260611075358No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA2420.7416260611075358No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT2350.7201740676044253No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT2290.701786644601759No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT2240.6864637920995372No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT2200.6742055100977598No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT1960.600655818087095No Hit
TTCGAGCCTCAGCGTCAGTTACACTCCGGACATATGCCTTCGCGATCGGA1950.5975912475866507No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACCGGT1880.5761392540835403No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1870.5730746835830959No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT1760.5393644080782078No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT1710.524041555575986No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT1670.5117832735742085No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1570.4811375685697649No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT1530.4688792865679875No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1490.45662100456621No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT1390.42597529956176644No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1310.4014587355582115No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT1270.38920045355643407No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTCCGGTAAGCTGCCTTCGCAATCGGA1250.38307131255554533No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT1210.3708130305537679No Hit
TTCGTGTCTCAGCGTCAGTTGTGGTCCAGTAAACCGCCTTCGCCACCGGT1180.36161931905243483No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT1120.3432318960497686No Hit
TTCGTGCATCAGCGTCAATTCTAGACCAGTAAGCCGCCTTCGCCACTGGT1120.3432318960497686No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA1100.3371027550488799No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1090.33403818454843553No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT1030.3156507615457694No Hit
TTCGAGTCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT1020.312586191045325No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT1010.30952162054488064No Hit
TTCGCGCCTCAGCGTCAATACCGGTCCAGACAGCCGCCTTCGCCACTGAT880.2696822040391039No Hit
TTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT850.26048849253777084No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT830.2543593515368821No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCTAGTGAGCTGCCTTCGCAATCGGA810.2482302105359934No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGGAAGCTGCCTTCGCAATCGGA810.2482302105359934No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT790.24210106953510466No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT770.23597192853421595No Hit
TTCGAGCCTCAACGTCAGTTACAGCTTAGCAGGCTGCCTTCGCAATCGGG740.22677821703288287No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT730.22371364653243847No Hit
TTCGTGCTTCAGCGTCAGTTATAGTATGGTATGCTGCCTTCGCAATCGGA730.22371364653243847No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGG660.20226165302932794No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGAAAGCCGCCTTCGCCACTGGT660.20226165302932794No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT650.19919708252888355No Hit
TTCGCGCCTCACCGTCAGTTACCGTCCAGTAATCCGCCTTCGCCACTGGT650.19919708252888355No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGCAAGCTGCCTTCGCCATCGGA650.19919708252888355No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGTAGATCGCCTTCGCCACTGGT620.1900033710275505No Hit
TTCGAGCCTCAGCGTCAGTTGCACTCCGGACATATGCCTTCGCGATCGGA620.1900033710275505No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT610.18693880052710612No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA560.1716159480248843No Hit
TTCGTGCCTCAACGTCAGTTATTGTCCAGAAAGTCGCCTTCGCCACCGGT550.16855137752443994No Hit
TTCGTCTTTCAGAGTCAGTTTCCGTCCAGCAAGCTGCCTTCGCTATTGGT540.1654868070239956No Hit
TTCGAGCATTAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGA530.16242223652355123No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAATCGCCTTCGCCACTGGT510.1562930955226625No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT490.15016395452177375No Hit
TTCGTGCCTCAGCGTCAGTAATAGCTTGGAAAGCTGCCTTCGCAATCGGT480.1470993840213294No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGACCGCCTTCGCCACTGGT480.1470993840213294No Hit
TTCGTGCCTCAACGTCAGTTTCTGTCCAGAAAGTCGCCTTCGCCACTGGT480.1470993840213294No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT460.1409702430204407No Hit
TTCGCCTCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT450.13790567251999633No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT450.13790567251999633No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT430.1317765315191076No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT430.1317765315191076No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT420.12871196101866322No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGCAGGCCGCCTTCGCCACTGGT410.12564739051821885No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT410.12564739051821885No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT390.11951824951733014No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGT390.11951824951733014No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT390.11951824951733014No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG360.11032453801599706No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGAGAGCCGCCTTCGCAACTGGT350.1072599675155527No Hit
TTCGTGTCTCAGCGTCAGTTATGGTCCAGTAAACCGCCTTCGCCACTGGT350.1072599675155527No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATAGGA340.10419539701510834No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT340.10419539701510834No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGA330.10113082651466397No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT330.10113082651466397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)