FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32631
Sequences flagged as poor quality0
Sequence length200
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA498515.276883944715147No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT17725.430418926787411No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT11083.395544114492354No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTTTCTTAAGTCAGCG10143.1074744874505837No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGCCGTTAAGTCAGCT9122.794888296405259No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG9042.7703717324017036No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT7702.3597192853421594No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT6501.9919708252888357No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT6101.8693880052710614No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT5381.6487389292390673No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT5231.6027703717324018No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT4911.504704115718182No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTGAGACAAGTCTGAA4891.4985749747172934No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTTGGGA4331.326959026692409No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT4281.3116361741901874No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGC4041.2380864821795226No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAGATAAGTTAGTT3571.0940516686586375No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA3381.0358248291501946No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCTTAAGTCAGGT3361.0296956881493058No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGCGATCAAGTTGGAA3190.9775979896417517No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA3180.9745334191413074No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT3060.937758573135975No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT3050.9346940026355307No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT2800.8580797401244216No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA2680.8213048941190891No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAA2640.8090466121173118No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCGGCGGCGTAAGTCAGTT2590.7937237596150899No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG2490.7630780546106464No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCG2460.7538843431093133No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA2240.6864637920995372No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT2190.6711409395973155No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGGAGATTAAGCGTGTT2100.6435598050933162No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA1900.582268395084429No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT1790.5485581195795409No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCCCAAGTCCCTC1770.5424289785786522No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG1690.5179124145750973No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAATTAAGTTAGTT1680.5148478440746529No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCGTTAAGTCAGCT1600.490331280071098No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA1570.4811375685697649No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA1510.4627501455670988No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCTGTAAGATAGAT1480.45355643406576573No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGTGATCAAGTCAGCT1460.44742729306487694No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGCAGCAAGTCTGAT1400.4290398700622108No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA1400.4290398700622108No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT1330.4075878765591003No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGAT1290.3953295945573228No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA1260.38613588305598967No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCCGTCCTTTAAGCGTGCT1230.37694217155465665No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT1220.37387760105421225No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGAGCAAGTCAGAA1190.36468388955287917No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT1160.3554901780515461No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA1140.34936103705065735No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGTTGTTAAGTCAGTT1090.33403818454843553No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGCAAGTCAGAA1080.3309736140479912No Hit
TCCGGAATTATTGGGCGTAAAGCGTGCGTAGGCGGCCACTTAAGTCTGTC1060.32484447304710246No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCTGATAAGTTGGAT1040.3187153320462137No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGTCGGCAAGTCAGAT1030.3156507615457694No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGACAAGTTGGAA990.3033924795439919No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGGA980.3003279090435475No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT980.3003279090435475No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGCAGGCAAGTCAGGC980.3003279090435475No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT970.29726333854310316No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT950.2911341975422145No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT900.27581134503999266No Hit
TCCGGAATTACTGGGTGTAAAGGGTGTGTAGGCGGGGTGTCAAGTCAGAT900.27581134503999266No Hit
ATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTTGTTTTGTAAGTCAGAG860.2635530630382152No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGTGGCAGATCAAGTCTGGA840.25742392203732645No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA840.25742392203732645No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT830.2543593515368821No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA820.2512947810364377No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCTGAA820.2512947810364377No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA810.2482302105359934No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT790.24210106953510466No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGCAAGTTGGAA770.23597192853421595No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGTCAGAT740.22677821703288287No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA720.22064907603199413No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCTTATAAGTCTGAT720.22064907603199413No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGCACGCCAAGCCAGAT700.2145199350311054No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA700.2145199350311054No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTTGCAAGTCTGAC690.21145536453066102No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGTGATTAAGTCAGCG690.21145536453066102No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGAATGCAAGTCAGAT680.20839079403021668No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGTAAGTTGGAA680.20839079403021668No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGGGATTAAGTCAGTT680.20839079403021668No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT670.20532622352977228No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAA660.20226165302932794No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCAAGCAAGTCAGAA640.1961325120284392No Hit
TCCGGAATTATTGGGCGTAAAGAGTGCGTAGGTGGTTACCTAAGCAGGGG630.19306794152799484No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGAT630.19306794152799484No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG610.18693880052710612No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGGATGCAAGTCAGAT570.17468051852532868No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGACTGTCAAGTCAGTC570.17468051852532868No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTTGCAAGTCTGAT550.16855137752443994No Hit
TTCGGATTTATTGGGCGTAAAGGGTCTGTAGGAGGTTCGTTAAATACGAG540.1654868070239956No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG540.1654868070239956No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA540.1654868070239956No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGGATACAAGTCAGAT520.15935766602310686No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGACAACAAGTTAGTT520.15935766602310686No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCCTTGTGGGTCAGGC520.15935766602310686No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGGA520.15935766602310686No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCTGTTTTTTAAGTTAGAG490.15016395452177375No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTAAGACAAGTCAGAA480.1470993840213294No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTAAGCAAGTCAGAA480.1470993840213294No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCATGCAAGTCAGAT480.1470993840213294No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTACGGCAAGCCAGAT480.1470993840213294No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAAATAAGTTAGTT460.1409702430204407No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGAA450.13790567251999633No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGATGGCAAGTCAGAT440.13484110201955196No Hit
TCCGGATTTATTGGGTTTAAAGGGAGTGTAGATGGATGTTTAAGTCAGTT430.1317765315191076No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGATTGGCAAGTTGGGA420.12871196101866322No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGACAAGTCTGAA420.12871196101866322No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTCAGAT400.1225828200177745No Hit
TCCGGAATTATTGGGTTTAAAGGGTCCGCAGGCGGACAGGTAAGTCAGTA370.11338910851644143No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGGCAAGTCTGAT370.11338910851644143No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT370.11338910851644143No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGATTAGTAAGTTAGAG370.11338910851644143No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGTTATGTAAGATAGCG360.11032453801599706No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTCGGAGATTAAGCGTGTT360.11032453801599706No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT360.11032453801599706No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCAGGGCAAGTCAGAT330.10113082651466397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)