FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39572
Sequences flagged as poor quality0
Sequence length200
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGTCTTATAAGCGTGTT575914.553219448094612No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT28157.113615687860103No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT26766.7623572222783785No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT21225.362377438592945No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA20255.117254624481957No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGAT20105.0793490346709795No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA11983.027393106236733No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA9132.307186899828161No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA8002.0216314565854643No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT6681.6880622662488627No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT6021.521277671080562No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTCTGGCAAGTCTGAT5641.4252501768927526No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT5501.3898716264025068No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT5361.3544930759122613No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA4891.235722227837865No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGAT4201.061356514707369No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT4161.0512483574244416No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT4041.0209238855756595No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCTGAA3760.9501667845951683No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA3400.8591933690488224No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGATGGCATGGCAAGTCTGAA3230.8162337005963813No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT3210.8111796219549177No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT3180.8035985039927221No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT2990.7555847568988173No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT2870.7252602850500354No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGAGCAAGTCTGAT2260.5711108864853937No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACAGCAAGTCTGAT2200.5559486505610027No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT2170.5483675325988072No Hit
CTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT2110.5332052966744163No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT2080.5256241787122208No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA1970.4978267461841706No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTGCTTAAGTCAGTT1920.48519154958051147No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT1860.47002931365612044No Hit
TCCGGAATTACTGGGTGTAAAGGGAGTGTAGGCGGGATGTCAAGTCAGAA1770.447285959769534No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATCACAAGTCAGAA1740.43970484180733854No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG1730.43717780248660676No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA1630.4119074092792884No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGTTTTTTAAGTCTGGA1620.4093803699585566No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT1570.39674517335489734No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA1500.3790558981097746No Hit
TCCGGAATGACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTCAGAT1480.37400181946831096No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGCAGCAAGTCTGAT1440.3638936621853836No Hit
CTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGAT1420.3588395835439199No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA1330.3360962296573335No Hit
TCCGGAATAATTGGGCGTAAAGGGCGCGTAGGCGGCTCGGTAAGTCTGGA1320.33356919033660165No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCTGTAAGATAGAT1300.328515111695138No Hit
TCCGGAATTACTGGGTGTAAAGGGAGTGCAGGCGGGACTGCAAGTCAGAA1290.32598807237440613No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGTCAGAT1260.3184069544122107No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT1260.3184069544122107No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGTAAGTTGGAA1180.298190639846356No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGTATCAAGTCTGAT1160.29313656120489234No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT1070.27039320731830585No Hit
TCCGGAATCATTGGGCGTAAAGAGTTCGTAGGTGGTTTGTTAAGTTTGGT1040.2628120893561104No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAGACCAAGTCTGAA1040.2628120893561104No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGTCAAGCGGGGT1020.2577580107146467No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA1020.2577580107146467No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA1010.2552309713939149No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT1010.2552309713939149No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT970.24512281411098757No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGTTGTTAAGTCAGTT970.24512281411098757No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT940.2375416961487921No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTAAGCAAGTCAGAA940.2375416961487921No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAGGTAAGTTAGTT860.2173253815829374No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA800.20216314565854646No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG790.19963610633781464No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA760.19205498837561913No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG720.1819468310926918No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAA660.16678459516830083No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCAAGCAAGTCAGAA650.164257555847569No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGATTTCTAAGTCAGCG640.16173051652683715No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGATTTGTAAGATAGTG640.16173051652683715No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG640.16173051652683715No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT620.1566764378853735No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGCAAGTTGGAA610.15414939856464166No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAATTAAGTTAGTT590.149095319923178No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGAA560.14151420196098252No Hit
TTCGGAATTACTGGGCGTAAAGGGAGTGTAGGCGGTTATGTAAGATAGTG550.13898716264025068No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGACAAGTCTGAA550.13898716264025068No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA540.13646012331951884No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGTGGTTTCTTAAGTCAGAG530.133933083998787No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACATGTAAGTCAGTT530.133933083998787No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT520.1314060446780552No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGAATGCAAGTCAGAT490.12382492671585969No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGCACGCCAAGCCAGAT480.12129788739512787No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATTGCAAGCCAGAT470.11877084807439604No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA450.11371676943293238No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGACTGTCAAGTCAGTC450.11371676943293238No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGGCAAGTCTGAT420.10613565147073688No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG410.10360861215000505No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT400.10108157282927323No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA400.10108157282927323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)