FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25430
Sequences flagged as poor quality0
Sequence length200
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA342813.480141565080613No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT15055.91820684231223No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTT13415.273299252850963No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT13395.265434526150217No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT11644.577270939834841No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGATTTGTAAGATAGTG10414.093590247738891No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA10254.030672434132914No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG8783.4526150216279987No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT5282.0762878489972474No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA5071.9937082186394024No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA4131.6240660637042863No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT4081.6044042469524185No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT4061.5965395202516712No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT4051.5926071569012976No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA3631.4274478961856076No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGTATCACAAGTCAGAA3201.2583562721195438No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA2971.1679119150609516No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT2951.1600471883602046No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT2681.053873377900118No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT2520.9909555642941407No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT2450.9634290208415257No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACATGTAAGTCAGTT2350.92410538733779No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT2290.9005112072355486No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA2180.8572552103814391No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTTCTAAGTCCATC2180.8572552103814391No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT1910.7510813999213528No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCTGTAAGACAGAT1800.7078254030672434No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGTAAGTTGGAA1730.6802988596146283No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG1600.6291781360597719No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG1550.6095163193079041No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT1440.5662603224537948No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGCGATCAAGTTGGAA1430.5623279591034211No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTCTGGCAAGTCTGAT1420.5583955957530475No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT1420.5583955957530475No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT1340.526936688950059No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT1240.4876130554463233No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAA1210.4758159653952025No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAA1190.4679512386944554No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCT1160.45615414864333464No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT1140.44828942194258753No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA1070.42076287848997246No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAAGGCAAGTCAGAT1060.4168305151395989No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT1060.4168305151395989No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA960.37750688163586316No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA950.37357451828548954No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA890.3499803381832481No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG870.342115611482501No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTCAAGCAAGTCAGAA750.2949272512780181No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA740.2909948879276445No Hit
TCCGGATTTATTGGGCGTAAAGCGTGTGTAGGCGGTTTGTTAAGTTTAAG690.27133307117577665No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG670.2634683444750295No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGCAGCAAGTCTGAT660.2595359811246559No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA660.2595359811246559No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGGCAAGTCTGAT640.25167125442390875No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG630.24773889107353517No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTCAGAT560.22021234762092018No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT560.22021234762092018No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTATGGCAAGTCAGAA560.22021234762092018No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT540.212347620920173No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA530.20841525756979945No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGACAAGTCTGAA510.2005505308690523No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT500.19661816751867872No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT500.19661816751867872No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTAAGCAAGTCAGAA490.19268580416830516No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT470.184821077467558No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGGA460.18088871411718443No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTAGTGCAAGTCAGAT430.1690916240660637No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA430.1690916240660637No Hit
TCCGGAATAATTGGGCGTAAAGGGCGCGTAGGCGGCTCGGTAAGTCTGGA400.15729453401494298No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTATGGCAAGTCTGAT390.1533621706645694No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAGATAAGTTAGTT370.14549744396382225No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTTGGGA370.14549744396382225No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT360.1415650806134487No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT350.1376327172630751No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGCAAGTTGGAA350.1376327172630751No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGTGGTTTATTAAGTCTGGT330.12976799056232796No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGAT330.12976799056232796No Hit
TTCGGAATTACTGGGCGTAAAGGGAGTGTAGGCGGTTATGTAAGATAGTG300.11797090051120723No Hit
TCCGGAATTATTGGGCGTAAAGAGTACGTAGGCGGTTTGCTAAGCGCAAG300.11797090051120723No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT290.11403853716083366No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG270.1061738104600865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)