FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51918
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT633912.209638275742517No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT41918.07234485149659No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA28555.499056204014022No Hit
TTCGTGCCTCAACGTCAGATATAGCTTGGTAAGCTGCCTTCGCAATCGGT24464.7112754728610495No Hit
TTCGAGCATGAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGA17943.4554489772333294No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT14222.7389344735929737No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA12862.476982934627682No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGTCGCCTTCGCCACCGGT11682.2497014522901497No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT11312.178435224777534No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT9111.7546900882160328No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT9091.7508378597018375No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT8891.712315574559883No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT7801.5023691205362302No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT5411.0420278130898726No Hit
TTCGAACCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACTGGT5351.030471127547286No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT5311.0227666705188951No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT5060.974613814091452No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACCGGT4890.9418698717207905No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT4840.9322393004353018No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT4810.9264609576640086No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT4290.8263030162949266No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT4240.816672445009438No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT4210.8108941022381447No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA4130.7954851881813629No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT3980.766593474324897No Hit
TTCGAGCATCAGTGTCAGTAACAGTCTAGTGAGCTGCCTTCGCAATCGGA3840.7396278747255287No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT3510.6760661042413036No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT3440.6625833044416195No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT3380.6510266188990331No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT3350.6452482761277398No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAGTCGCCTTCGCCACTGGT3280.6317654763280558No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT3110.5990215339573943No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT3050.587464848414808No Hit
TTCGTGCCTCAACGTCAGCTATAGCTTGGTAAGCTGCCTTCGCAATCGGT3030.5836126199006125No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT3030.5836126199006125No Hit
TTCGTGCCTCAGCGTCCGATATAGATTTGTAAGCTGCCTTCGCAATCGGT2940.566277591586733No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT2880.5547209060441465No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT2700.5200508494163874No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT2640.5084941638738011No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA2640.5084941638738011No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT2600.5007897068454101No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT2580.49693747833121465No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT2560.4930852498170191No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT2510.4834546785315305No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT2490.47960245001733504No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT2290.4410801648753804No Hit
TTCGCGCCTCAGCGTCAATACCGGTCCAGACAGCCGCCTTCGCCACTGAT2280.43915405061828267No Hit
TTCGTGCATGAGCGTCAGTTTTGCCCCAGGGGGCTGCCTTCGCCATCGGT2280.43915405061828267No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA2240.4314495935898917No Hit
TTCGTGATTCAGCGTCAGTTGTGGTCCAGTAATCCGCCTTCGCCACTGAT2180.41989290804730534No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT2110.4064101082476212No Hit
TTCGAGCCTCAATGTCAGTTGCGGCTTAGCAGGCTGCCTTCGCAATCGGA2040.3929273084479371No Hit
TTCGAGCATCAGCGTCAGTTACAATCCAGTAAGCTGCCTTCGCAATCGGA1860.358257251820178No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCTCTGGT1840.35440502330598256No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGAAGGCTGCCTTCGCCATCGGT1820.350552794791787No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1810.3486266805346893No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT1790.34477445202049384No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT1770.34092222350629836No Hit
TTCGTGCTTCAGCGTCAGTTGCAGACCAGGCGACCGCCTTCGCCACTGGT1770.34092222350629836No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG1630.31395662390693013No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT1590.30625216687853923No Hit
TTCGTGCATCAGCGTCAATTCTAGACCAGTAAGCCGCCTTCGCCACTGGT1580.3043260526214415No Hit
TTCGTGCCTCAGCGTCAGTAACGGCCCAGCAAACCGCTTTCGCCACTGGT1500.28891713856465967No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1470.2831387957933665No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT1430.27543433876497553No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT1420.2735082245078778No Hit
TTCGAGCATTAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGA1390.26772988173658463No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA1360.26195153896529144No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT1350.2600254247081937No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT1290.24846873916560733No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT1240.23883816788011863No Hit
TTCGTGTCTCAGCGTCAGGTATCGTCCAGTAACTCGCCTTCGCCACTGGT1190.22920759659463003No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT1180.22728148233753226No Hit
TTCGCGCCTCAGCGTCAGTGACGGCCCAGAGAGCTGCCTTCGCCATCGGT1140.21957702530914133No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT1080.20802033976655496No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT1050.20224199699526174No Hit
TTCGCATCTCAGCGTCAGTTACGAGCCAGATAGCCGCCTTCGCCACTGAT1010.19453753996687084No Hit
TTCGCCCCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT1000.1926114257097731No Hit
TTCGACCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACTGGT990.19068531145267537No Hit
TTCGCGCCTCAGCGTCAGTTTCGGCCCAGAGAGCTGCCTTCGCCGTCGGT920.17720251165299128No Hit
TTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAGCCGCCTTCGCCACTGGT910.1752763973958935No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT880.16949805462460033No Hit
TTCGTCCCTGAGTGTCAGTAACCGTCCAGAAATCTGCCTTCGCTTTCGAT850.16371971185330714No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT840.16179359759620943No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT800.15408914056781847No Hit
TTCGCGCCTCAGCGTCAGTTCCGGCCCAGAGGACTGCCTTCGCCATCGGT800.15408914056781847No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT790.15216302631072076No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT770.14831079779652528No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT750.14445856928232984No Hit
TTCGTGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACCGGT730.14060634076813436No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA720.13868022651103662No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT690.13290188373974343No Hit
TTCGTGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACTGGT610.11749296968296158No Hit
TTCGTGTCTCAGCGTCAGTTATGGTCCAGTAAACCGCCTTCGCCACTGGT590.11364074116876613No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGTAGGCCGCCTTCGCCACTGGT550.10593628414037522No Hit
TTCGCATATGAGCGTCAGTATTTGCCCAGGAGACTGCCTTCGCCTTTGGT530.10208405562617975No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGACCGCCTTCGCCACTGGT530.10208405562617975No Hit
TTCGCACCTTAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT530.10208405562617975No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT530.10208405562617975No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT520.10015794136908202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)