FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51918
Sequences flagged as poor quality0
Sequence length200
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA527710.164104934704726No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTAGATAAGTTAGAG40637.825802226588081No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT30665.905466312261643No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTCAGATAAGTTAGAG26805.161986209021919No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCGTTAAGTCAGCT18413.5459763473169232No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT16843.243576408952579No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTGAGACAAGTCTGAA13322.5655841904541776No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTTCTAAGTCCATC9831.8933703147270695No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT9091.7508378597018375No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT8891.712315574559883No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT8801.6949805462460035No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT8331.60445317616241No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG7771.496590777764937No Hit
TCCGGATTTATTGGGCGTAAAGGGAACGCAGGCGGTCTTTTAAGTCTGAT5661.0901806695173157No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT5201.0015794136908203No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT5030.9688354713201588No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGCGATCAAGTTGGAA4870.938017643206595No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGTGCAAGTCAGAA4790.9226087291498132No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA4720.909125929350129No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT4270.8224507877807312No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA4140.7974113024384607No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG4050.780076274124581No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAGGTAAGTTAGTT3900.7511845602681151No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA3510.6760661042413036No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT3360.6471743903848377No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT3350.6452482761277398No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCGATTAAGTTAGAT3300.6356177048422512No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG3150.6067259909857853No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCAGATTAAGTTAGAG3110.5990215339573943No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT2990.5759081628722216No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA2960.5701298201009284No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT2830.5450903347586579No Hit
TCCGGAATCACTGGGCGTAAAGGGCGCGTAGGTGGTCTGTTAAGTCAGAT2740.5277553064447783No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGAATGCAAGTCAGAT2690.5181247351592896No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGACTGCAAGTTGGAT2620.5046419353596056No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG2610.5027158211025078No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA2590.49886359258831237No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT2580.49693747833121465No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA2580.49693747833121465No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGACGGCAAGTCAGAT2540.4892330213028237No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT2470.47575022150313956No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAATACAAGTTAGTT2400.46226742170345547No Hit
TCCGGAATTACTGGGTGTAAAGGGCGTGTAGGCGGAGCTGCAAGTCAGAT2370.45648907893216223No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA2350.4526368504179668No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATCACAAGTCAGAA2330.44878462190377133No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTTCTAAGTCTATC2300.4430062791324781No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT2180.41989290804730534No Hit
ATCGGAATTACTGGGCGTAAAGCGCGCGTAGGTTGTTTTGTAAGTCAGAG2030.3910011941908394No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG2010.387148965676644No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCG2000.3852228514195462No Hit
TCCGGATTTACTGGGTGTAAAGGGTGAGTAGGCGGGTCTGCAAGTCAGAT1980.38137062290535073No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTT1930.37174005161986207No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGAGAGACAAGTCAGAT1870.3601833660772757No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTTTCTTAAGTCAGCG1820.350552794791787No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCT1730.33321776647790746No Hit
TCCGGAATGACTGGGCGTAAAGGGAGCGTAGGCGGCACAACAAGTTAGGA1720.33129165222080975No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGCGGCCCGTTAAGTCTGGA1620.3120305096498324No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG1580.3043260526214415No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGTCAAGCGGGGT1520.29276936707885515No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGCGGATTAAGTTGGAT1510.2908432528217574No Hit
TCCGGAATTATTGGGCGTAAAGCGTGCGTAGGCGGCCACTTAAGTCTGTC1480.2850649100504642No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT1460.2812126815362687No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGGATACAAGTCAGAT1400.26965599599368234No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT1380.26580376747948686No Hit
TCCGGATTTATTGGGCGTAAAGCGTGTGTAGGCGGTTTATTAAGTTTAAG1340.25809931045109596No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT1300.25039485342270507No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGAT1270.24461651065141185No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA1260.2426903963943141No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA1200.23113371085172774No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA1100.21187256828075043No Hit
TCCGGATTTACTGGGTGTAAAGGGTGTGTAGGCGGGACTGCAAGTCAGAT1060.2041681112523595No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA1020.19646365422396855No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGAT1010.19453753996687084No Hit
ATCGGAATTACTGGGCGTAAAGCGCGTGTAGGCTGTACGGCAAGTTGGAG1000.1926114257097731No Hit
TCCGGAATGATTGGGCGTAAAGCGCGCGCAGGCGGCCGCTCAAGCGGGAC980.18875919719557763No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGAGCGCTAAGCGGGAC960.18490696868138218No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAAGGCAAGTCAGAT950.18298085442428444No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGACTGGCAAGTCAGTG950.18298085442428444No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT940.18105474016718673No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT930.179128625910089No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT930.179128625910089No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT910.1752763973958935No Hit
TTCGGATTCATTGGGCGTAAAGGGTCCGCAGGTGGTTCGATAAGTCTGAC890.17142416888169806No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGGACAAGTTGGAA870.1675719403675026No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTTGGGA870.1675719403675026No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT870.1675719403675026No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA860.16564582611040488No Hit
CTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT860.16564582611040488No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT840.16179359759620943No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTGCTTAAGTTGGAT800.15408914056781847No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA800.15408914056781847No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGCAGGCGGCCTGCCAAGCGGGAT790.15216302631072076No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA770.14831079779652528No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGCAAGTTGGAA760.14638468353942757No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGCAAGTCAGAA730.14060634076813436No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGGTAAGTTTGAA670.12904965522554798No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGATGGCTAAGTCTGAT670.12904965522554798No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCAACTAAGTCTGGA650.12519742671135253No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTATTAAGTCAGTT640.12327131245425478No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTTGCAAGTCTGAT590.11364074116876613No Hit
TCCGGAATGACTGGGCGTAAAGGGCGCGTAGGCGGCTGTGTAAGTCTGGA580.1117146269116684No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA570.10978851265457067No Hit
TCCGGAATGATTGGGCGTAAAGGGTACGTAGGCGGCCAGATAAGTCTGGT560.10786239839747293No Hit
TCCGGAATGACTGGGCGTAAAGGGTGTGTAGGCGGGCTCGCAAGTTGGAT560.10786239839747293No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT530.10208405562617975No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG530.10208405562617975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)