FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44612
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA955521.41800412445082No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT37258.34977136196539No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA30686.877073433156998No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT26916.032009324845333No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT24295.444723392809109No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT21634.848471263337219No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT14403.2278310768403125No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG11922.671926835828925No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA11642.609163453779252No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT10142.27293104994172No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT8681.9456648435398547No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT7771.7416838518784183No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA7731.732717654442751No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA6211.3920021518873846No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT5931.3292387698377117No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT5111.1454317224065274No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT4911.10060073522819No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT4571.0243880570250157No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT3960.8876535461310857No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT3420.7666098807495741No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT3340.748677485878239No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT3240.7262619922890702No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCTAGTGAGCTGCCTTCGCAATCGGA2910.652290863444813No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT2800.6276338204967273No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA2770.6209091724199767No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT2240.5021070563973818No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT2230.499865507038465No Hit
TTCGTGCTTCAGCGTCAGTTATAGTATGGTATGCTGCCTTCGCAATCGGA2090.46848381601362865No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT2030.45503451986012733No Hit
TTCGAGTCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT2000.44830987178337667No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1950.4371021249887923No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT1930.4326190262709585No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1850.4146866313996234No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT1690.37882184165695326No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT1670.3743387429391195No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT1620.3631309961445351No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT1360.30485071281269616No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT1350.30260916345377925No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT1330.2981260647359455No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT1160.2600197256343585No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1140.2555366269165247No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGTAGGCCGCCTTCGCCACTGGT1140.2555366269165247No Hit
TTCGTTCCTCAGTGTCAATTACGGCCCAGTAAAGCGCTTACGCCACCGAT1010.2263964852506052No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA930.20846409037927016No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGACCGCCTTCGCCACTGGT860.19277324486685196No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT860.19277324486685196No Hit
TTCGTGCTTCAGCGTCAGTTATAGCCCAGTATGCTGCCTTCGCAATCGGG830.1860485967901013No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT820.18380704743118445No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT750.16811620191876625No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT720.1613915538420156No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGCAGGCTGCCTTCGCCATCGGT710.15915000448309874No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT700.15690845512418183No Hit
TTCGCGCCTCACCGTCAGTTGCCGTCCAGTTATCCGCCTTCGCCACTGGT690.15466690576526496No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT680.15242535640634808No Hit
TCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGAG680.15242535640634808No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT670.15018380704743117No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT660.1479422576885143No Hit
TTCGAGCATCAGTGTCAGTAACAGTCTAGTGAGCTGCCTTCGCAATCGGA650.14570070832959742No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGA630.14121760961176363No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCTACTGGT620.13897606025284678No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGAAAGCCGCCTTCGCCACTGGT620.13897606025284678No Hit
TTCGTGCCTGAGCGTCAGTTATGTCCCAGGAGGCTGCCTTCGCCATCGGT600.134492961535013No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT590.13225141217609612No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT560.12552676409934546No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT540.12104366538151169No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGGT530.11880211602259483No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT510.11431901730476104No Hit
TTCGAGCCTCAGCGTCATTCACCGTCCAGTAAGCCGCCTTCGCCACTGGT480.10759436922801041No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGT460.10311127051017663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)