FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44612
Sequences flagged as poor quality0
Sequence length200
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT941321.099704115484624No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT38098.538061508114408No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT31897.148300905585941No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA29656.646193849188559No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT23825.339370572940016No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG23245.209360710122836No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG11542.5867479601900834No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG10592.37380077109298No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT8521.9098000537971844No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT8341.8694521653366807No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATGCTTAAGTCAGTT8281.8560028691831794No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT7051.5802922980364027No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT6741.5108042679099793No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT6091.365103559580382No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGGA5511.2350936967632027No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA4611.0333542544606833No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT4420.9907648166412625No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT4100.9190352371559222No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT3370.7554021339549897No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA3320.7441943871604052No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT3270.7329866403658208No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT2870.6433246660091455No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT2870.6433246660091455No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGC2850.6388415672913117No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT2540.5693535371648883No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT2490.5581457903703039No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCG2160.48417466152604677No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG2050.4595176185779611No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCTGTAAGATAGAT2010.4505514211422935No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGTGATTAAGTCAGCG1800.40347888460503906No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT1790.40123733524612215No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA1680.3765802922980364No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT1570.35192324934995073No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT1530.34295705191428316No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGAGCAAGTCAGAA1340.30036761409486235No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGAT1320.2958845153770286No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA1250.2801936698646104No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT1190.2667443737111091No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT1120.2510535281986909No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA1030.230879583968439No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGG990.22191338653277143No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCGATGCAAGTCTGGA980.21967183717385455No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGAGACAAGTTGAAT940.21070563973818704No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG940.21070563973818704No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA940.21070563973818704No Hit
TCCGGAATCATTGGGCGTAAAGAGTTCGTAGGTGGCATGTAAAGTCTGGT910.20398099166143638No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAA900.20173944230251953No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT820.18380704743118445No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT810.18156549807226754No Hit
TCCGGAATGACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTCAGAT790.1770823993544338No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGAAGAATAAGTCAGCG770.17259930063660003No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTAGCAAGTCTGAA740.16587465255984937No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCCGGCAAGTCAGAT730.1636331032009325No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAA720.1613915538420156No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA710.15915000448309874No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCCCGGTAAGTCAGGG700.15690845512418183No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGCAAGTTGGAA650.14570070832959742No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA640.14345915897068054No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCGCCTAAGCGGAAC600.134492961535013No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT550.12328521474042858No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT530.11880211602259483No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT520.11656056666367794No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGGA520.11656056666367794No Hit
TCCGGATTTACTGGGCGTAAAGGGTGCGTAGGCGGTCTTTTAAGTCAGGA520.11656056666367794No Hit
ATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGTTGAGTAAGACAGAT510.11431901730476104No Hit
CCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTG510.11431901730476104No Hit
TCCGGAATTATTGGGCGTAAAGAGTGCGTAGGTGGTTACCTAAGCAGGGG490.10983591858692728No Hit
TCCGGATTTATTGGGTTTAAAGGGCGCGTAGGCGGGACGCCAAGTCAGCG480.10759436922801041No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGACAAGCCAGAT470.10535281986909352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)