FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30071
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT572819.048252469156328No Hit
TTCGAGCTTCAGCGTCAGTTGCGCTCCAGTGAGCTGCCTTCGCAATCGGA444014.765056034052742No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT391713.025838848059593No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT22267.402480795450766No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACCGGT9983.3188121445911345No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT7682.5539556383226367No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT6742.241362109673772No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT6582.1881547005420505No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT6222.068438029995677No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT5361.7824482059126734No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA4711.5662931063150545No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT4421.469854677263809No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA3871.2869542083735162No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT3751.2470486515247248No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT3571.187190316251538No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT3261.0841009610588275No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT3091.0275680888563732No Hit
TTCGAGCATGAACGTCAGTTACAGTTTAGCAAGCTGCCTTCGCAATCGGG2590.8612949353197433No Hit
TTCGTGCATGAGCGTCAGTCGCATCCCAGGGGGCTGCCTTCGCCATTGGT2150.7149745602075089No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1910.6351634465099265No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT1650.5487014066708789No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT1640.5453759436001463No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1430.4755412191147617No Hit
TTCGAGCTTCAGCGTCAGTTGCGCTCCAGTAAGCTGCCTTCGCAATCGGA1360.4522629776196336No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1340.44561205147816835No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGG1280.42565927305377277No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT1280.42565927305377277No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT1270.4223338099830401No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT1260.41900834691230754No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT1250.415682883841575No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA1200.39905556848791196No Hit
TTCGAGCCTCAGCGTCAGTTGCACTCCGGACATATGCCTTCGCGATCGGA1200.39905556848791196No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT1100.3658009377805859No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA1090.36247547470985336No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT1080.35915001163912075No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG1070.35582454856838813No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT930.30926806557813175No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT820.27268797180007315No Hit
TTCGGGAATGAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT810.26936250872934053No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT800.266037045658608No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT730.24275880416347978No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT700.23278241495128196No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT690.22945695188054935No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT690.22945695188054935No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT580.19287685810249075No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG570.18955139503175816No Hit
TTCGTGCCTCAACGTCAGTTATTGTCCAGAAAGTCGCCTTCGCCACCGGT500.16627315353662997No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT500.16627315353662997No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT470.15629676432443218No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGGCGGCCGCCTTCGCCACCGG440.14632037511223436No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT410.13634398590003657No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT410.13634398590003657No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT400.133018522829304No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT400.133018522829304No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT370.12304213361710618No Hit
TTCGAGCCTCAGCGTCAGTGCTGCCCCGGCACAATGCCTTCGCGATCGGA350.11639120747564098No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGTAGGCCGCCTTCGCCACTGGT350.11639120747564098No Hit
TTCGAACCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACTGGT340.1130657444049084No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT330.10974028133417578No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG320.10641481826344319No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGAGAGCCGCCTTCGCAACTGGT310.10308935519271058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)