FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32419
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTGCATCAGCGTCAGTCCCTGCTTCGTGAGATGCCTTCGCAATCGGT447913.815972115117678No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT25557.881180789043462No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT21476.622659551497579No Hit
TTCGAGCATCAACGTCAGTTACGGTCCAGCAAGCTGCCTTCGCAATCGGA15274.710200808168049No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT15094.654677812393967No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT9712.9951571609241494No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT6692.0636046762700886No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT6061.8692741910607977No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA5711.7613128103889695No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA5201.6039976556957338No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT4991.539220827292637No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT4641.4312594466208088No Hit
TTCGTGCCTCAGCGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGAT3791.169067522132083No Hit
TTCGAGCATCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCACTGGT3551.0950368610999721No Hit
TTCGTTCCTCAGCGTCAATAACGGCCCAGTGAAGCGCTTTCGCCACCGGT3551.0950368610999721No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT2950.9099602085196952No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT2890.8914525432616675No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT2590.798914216971529No Hit
TTCGCACTTTAGCGTCAGTAAAGGCCCAGAAATCCGCCTTCGCCACCGGT2490.7680681082081495No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT2370.7310527776920941No Hit
TTCGCGCCTCAGCGTCGATCCCGTGCTCGTGAGGTGCCTCCGCAATCGGC2370.7310527776920941No Hit
TTCGTGCATCAGCGTCAATTCTAGACCAGTAAGCCGCCTTCGCCACTGGT2260.6971220580523767No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT2150.6631913384126592No Hit
TTCGTGCTTCAGTGTCAGCAACAGACCAGACGGCCGCCTTCGCCACCGGT2000.61692217526759No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT1810.5583145686171689No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT1780.5490607359881551No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGAAGGCTGCCTTCGCCATCGGT1700.5243838489774515No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT1700.5243838489774515No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1700.5243838489774515No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT1670.5151300163484376No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA1630.5027915728430858No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT1610.4966223510904099No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGTAACCCGCCTTCGCCACTGGT1600.49353774021407204No Hit
TTCGCGCCTCAGCGTCAATACCGGTCCAGACAGCCGCCTTCGCCACTGAT1570.4842839075850582No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1540.4750300749560443No Hit
TTCGTGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACCGGT1500.4626916314506925No Hit
TTCGTGACTGAGCGTCAGTATCGAGCTAGTAAGCCGCCTTCGCCACTGGT1490.45960702057435454No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT1450.4472685770690027No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCTCTGGT1400.431845522687313No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT1360.4195070791819612No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT1360.4195070791819612No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT1360.4195070791819612No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT1360.4195070791819612No Hit
TTCGTTCCTCAGTGTCAATTACGGCCCAGTAAAGCGCTTACGCCACCGAT1330.4102532465529473No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT1270.3917455812949196No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT1270.3917455812949196No Hit
TTCGTGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1250.38557635954224373No Hit
TTCGCACATGAGCGTCAGTAGTGTGCCAGGAAGCCGCCTTCGCCACTGGT1140.3516456399025263No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGCAAGCTGCCTTCGCAATCGGA1140.3516456399025263No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1130.34856102902618835No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT1090.33622258552083656No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1060.3269687528918227No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1040.32079953113914683No Hit
TTCGAGCCTCAGCGTCAGTTGCACTCCGGACATATGCCTTCGCGATCGGA980.30229186588111906No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT970.2992072550047811No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT970.2992072550047811No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT930.28686881149942933No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA930.28686881149942933No Hit
TTCGAGCCTCAGCGTCAGTGCTGCCCCGGCACAATGCCTTCGCGATCGGA910.28069958974675346No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT850.26219192448872575No Hit
TTCGAGCATGAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGA820.2529380918597119No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTCCGGTAAGCTGCCTTCGCAATCGGA810.24985348098337393No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAGGCCGCCTTCGCCACTGGT790.24368425923069806No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT780.2405996483543601No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC750.23134581572534624No Hit
TTCGCGCCTCAGTGTCAGTCTATGTCCAGATAGCCGCCTTCGCCACTGGT750.23134581572534624No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT740.22826120484900828No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCTCTGGT710.21900737221999447No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT690.21283815046731855No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA690.21283815046731855No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAGTCGCCTTCGCCACTGGT690.21283815046731855No Hit
TTCGTGCCTCAGCGTCCGATATAGATTTGTAAGCTGCCTTCGCAATCGGT670.20666892871464265No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT650.20049970696196673No Hit
TTCGTGTCTCAGCGTCAGGTATCGTCCAGTAACTCGCCTTCGCCACTGGT650.20049970696196673No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT620.1912458743329529No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATAGGA620.1912458743329529No Hit
TTCGTCCCTCACCGTCGGATCCGTTCTAGCAAGACGCCTTCGCCACCGGT620.1912458743329529No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT610.18816126345661496No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT610.18816126345661496No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT600.185076652580277No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT580.1789074308276011No Hit
TTCGTGCATGAGCGTCAGTTTTGCCCCAGGGGGCTGCCTTCGCCATCGGT550.16965359819858725No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGACCGCCTTCGCCACTGGT510.15731515469323545No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT510.15731515469323545No Hit
TTCGTGCTTCAGCGTCAGTTATAGTATGGTATGCTGCCTTCGCAATCGGA510.15731515469323545No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT510.15731515469323545No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTGAATCGCCTTCGCCGCTGGT450.13880748943520774No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT450.13880748943520774No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT450.13880748943520774No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT440.1357228785588698No Hit
TTCGCCCTTTAGCGTCAGTAAAGGCCCAGAAATCCGCCTTCGCCACCGGT430.13263826768253184No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT430.13263826768253184No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG420.1295536568061939No Hit
TTCGAGCATCAGCGTCAGTTACAATCCAGTAAGCTGCCTTCGCAATCGGA410.12646904592985594No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGAGAGCCGCCTTCGCAACTGGT410.12646904592985594No Hit
TTCGTGCATCAGCGTCAGTCCCTGCTTCGTGAGCTGCCTTCGCAATCGGT400.12338443505351801No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT400.12338443505351801No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT380.1172152133008421No Hit
TTCGCGCCTCAGCGTCAGTTTCGGCCCAGAGAGCTGCCTTCGCCGTCGGT380.1172152133008421No Hit
TTCGAGCCTCAACGTCAGTCACTGTCCAGTAAGCCGCCTTCGCCACTGGT380.1172152133008421No Hit
TTCGTGCCTCAACGTCAGATATAGCTTGGTAAGCTGCTTTCGCAATCGGT380.1172152133008421No Hit
TTCGTGCCTGAGCGTCAGTTATGTCCCAGGAGGCTGCCTTCGCCATCGGT380.1172152133008421No Hit
TTCGTGCCTCAGCGTCCGTTGCACCCTAGCAGGCTGCCTTCGCAATCGGG360.11104599154816619No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGTAATCCGCCTTCGCCACTGGT360.11104599154816619No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT350.10796138067182826No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGGAAGCTGCCTTCGCAATCGGA350.10796138067182826No Hit
TTCGCTCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT350.10796138067182826No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT350.10796138067182826No Hit
TTCGTGTCTCAGCGTCAGTTGTCGTCCAGTAAACCGCCTTCGCCACTGGT340.1048767697954903No Hit
TTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAGCCGCCTTCGCCACTGGT340.1048767697954903No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT340.1048767697954903No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT340.1048767697954903No Hit
TTCGTGTCTCAGCGTCAGTTGTGGTCCAGTAAACCGCCTTCGCCACCGGT330.10179215891915235No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA330.10179215891915235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)