FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61522
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT915114.874353889665484No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT742112.062351679074153No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT615510.004551217450668No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT33575.456584636390235No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT32315.251779851110172No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT25514.146484184519359No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT24393.9644354864926368No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT17392.8266311238256234No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT17242.802249601768473No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT14432.3455024218978577No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT11341.8432430675205618No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT11081.8009817626215012No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT10911.7733493709567312No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT10411.69207763076623No Hit
TTCGTGCCTCAACGTCAGTTATTGTCTAGAAAGTCGCCTTCGCCACTGGT9051.4710184974480673No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT8521.3848704528461364No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAATCGCCTTCGCCACTGGT7601.2353304508956144No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT7451.2109489288384643No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT6191.006144143558402No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT5850.9508793602288613No Hit
TTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGCCGCCTTCGCCACTGGT4620.7509508793602289No Hit
TTCGAGCCTCAGCGTCAGTTGCAGACCAGAGAGCCGCCTTCGCCACTGGT3470.5640258769220766No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT3290.5347680504534963No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT3190.5185137024153961No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT3180.5168882676115861No Hit
TTCGTGCCTCAGCGTCAGTAACGGCCCAGCAAACCGCTTTCGCCACTGGT2980.48437957153538574No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT2960.4811287019277657No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT2940.4778778323201456No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT2860.4648743538896655No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT2820.45837261467442536No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT2410.39172978771821465No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT2350.3819771788953545No Hit
TTCGTGCCTCAGCGTCAGTAATGGCCCAGTAAACCGCCTTCGCCACTGGT1950.3169597867429538No Hit
TTCGAGCCTCAGTGTCAGTTACAGGCCAGAGAGCCGCTTTCGCCACCGGT1820.2958291342934235No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGACAGCCGCCTTCGCCACTGGT1790.29095282988199345No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT1580.25681869900198306No Hit
TTCGCACCTCAGCGTCAGTAATGGCCCAGAAAGCTGCCTTCGCCATCGGT1530.24869152498293293No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT1480.24056435096388284No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT1470.2389389161600728No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT1450.2356880465524528No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACCGGT1300.2113065244953025No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT1280.20805565488768246No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA1270.2064302200838724No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGAT1190.19342674165339227No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGCT1140.1852995676343422No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCCTTCGCCACTGGT1050.170670654400052No Hit
TTCGAGCATCAGCGTCAGTTACAATCCAGTAAGCTGCCTTCGCAATCGGA950.1544163063619518No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGCAGGCTGCCTTCGCCATCGGT930.15116543675433178No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT920.14954000195052175No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT890.1446636975390917No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA870.14141282793147167No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT850.1381619583238516No Hit
TTCGGGACTTAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT810.13166021910861156No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT790.12840934950099153No Hit
TTCGTACCTCAGTGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT790.12840934950099153No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA770.12515847989337148No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT760.12353304508956146No Hit
TTCGTGCTTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT730.11865674067813141No Hit
TTCGTGCCTCAGTGTCAGTACCAGTCCAGTAAGTCGCCTTCGCCACTGGT720.11703130587432137No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT710.11540587107051135No Hit
TTCGTCCATGACCGTCAGTGGTGGCCTAGCAAACCGCCTTCGCCACTGGT690.11215500146289134No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGAGAGCCGCTTTCGCCACCGGT670.10890413185527129No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGCAGGCCGCCTTCGCCACTGGT630.10240239264003122No Hit
TCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTG620.10077695783622118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)