FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50268
Sequences flagged as poor quality0
Sequence length200
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT707114.066603007877776No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG36087.177528447521286No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT35807.121827007241187No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT30186.0038195273334924No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT28165.601973422455638No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT19653.9090475053712104No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT15973.1769714331184846No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT15643.111323307074083No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT15363.055621866793984No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA12132.413065966419989No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT11962.3792472348213574No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT11282.2439723084268324No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG11142.216121588286783No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT10992.186281530993873No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG10912.170366833770988No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT8601.7108299514601732No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA7551.5019495504098035No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT6871.3666746240152783No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTATTAAGTCAGTT6451.2831224635951302No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT5561.1060714569905308No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT4430.8812763587172754No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT3810.7579374552399141No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT3680.7320760722527254No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT3600.71616137502984No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT3540.7042253521126761No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT3270.650513248985438No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCAACTAAGTCTGGA3160.6286305403039707No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGTGGTTTATTAAGTCTGGT3130.6226625288453888No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT2370.471472905227978No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT2270.45157953369937137No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCGCCTAAGCGGAAC2100.41776080210074No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT1980.39388875626641207No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAATTAAGTTAGTT1950.38792074480783No Hit
TCCGGAATAATTGGGCGTAAAGGGCGCGTAGGCGGCTCGGTAAGTCTGGA1930.3839420705021087No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT1900.37797405904352666No Hit
TCCGGAATTATTGGGCGTAAAGAGTACGTAGGTGGTTTTCTAAGCACGGG1630.3242619559162887No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA1550.3083472586934034No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA1540.3063579215405427No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT1540.3063579215405427No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGAGCAAGTCAGAA1530.30436858438768205No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGACAAGTCTGAT1460.29044322431765734No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGTGGTTTCTTAAGTCAGAG1450.2884538871647967No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGGA1270.2526458184133047No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGCCAAGTCAGCT1260.250656481260444No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGG1180.2347417840375587No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT1110.220816423967534No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGAATGCAAGTCAGAT1080.21484841250895204No Hit
TCCGGATTTATTGGGCGTAAAGCGCGCGTAGGCGGCTTGTTAAGCAAGGT1060.21086973820323068No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACATGTAAGTCAGTT990.19694437813320603No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCAGTGCGGCAAGTCTGAT960.190976366674624No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGAACTGCAAGTCAGTG910.18102968091032068No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCTGAA870.17307233229887803No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA850.16909365799315668No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG840.167104320840296No Hit
CTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT830.16511498368743535No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA820.16312564653457468No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGGAAGCAAGTCAGAT780.15516829792313203No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAA730.14522161215882867No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGAT670.1332855892416647No Hit
TCCGGAATTACTGGGTGTAAAGGGTGTGTAGGTGGTATGGCAAGTCAGAA650.12930691493594335No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGAT620.12333890347736133No Hit
TCCGGAATGACTGGGCGTAAAGGGCGTGTAGGCGGCCGATTAAGTGTGGA610.12134956632450068No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATAACAAGTCTGAT590.11737089201877934No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA570.113392217713058No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAA520.10344553194875468No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTATGGCAAGTCTGAT510.101456194795894No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA510.101456194795894No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)