FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33808
Sequences flagged as poor quality0
Sequence length200
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT1237336.597846663511596No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATAGCAAGTCTGGA621318.377307146237577No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGAT378011.180785612872693No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT22566.6729768102224325No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT18935.599266445811643No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGTGTAAGTCTGAT8842.614765735920492No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGCATGATAAGTCAGAA8602.543776620918126No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATTGGTCAGTCTGTC7272.150378608613346No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT5081.5026029342167533No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAAGCAAGTCTGAA2880.8518693800283956No Hit
CTCGGAATCACTGGGCGTAAAGGGCGCGTAGGCGGATTATCAAGTCTCTT2560.7572172266919073No Hit
TCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTA2380.7039753904401325No Hit
TCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGATGTTTAAGTGGGAT2030.6004495977283484No Hit
TCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGAT1400.4141031708471367No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT1070.3164931377188831No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGAT930.27508282063416944No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA850.25141978230004736No Hit
TCCGGATTTACTGGGCGTAAAGGGTGCGTAGGCGGTCTTTCAAGTCAGGA710.21000946521533365No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCAGCAAGGGTCTGTG700.20705158542356836No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATCAGTCAGTCTGTC570.16859914813061996No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT550.16268338854708944No Hit
CCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTG520.15380974917179366No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATCGGTCAGTCTGTC490.14493610979649788No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCATGATAAGTCAGAA400.1183151916706105No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGG370.10944155229531471No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATGTTTAAGTCAGTT360.10648367250354945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)