FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31741
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT461614.542705018745473No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA455414.347374058788317No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT414813.06827132100438No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA16045.053400964052803No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCTAGTGAGCTGCCTTCGCAATCGGA14564.587127059638953No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT13514.256324627453451No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT12523.9444251913928357No Hit
TTCGAGCCTCAGCGTCAGTTGCAGACCAGACAGCCGCCTTCGCCACTGGT11693.682933744998582No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG7452.347122018839986No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT7322.306165527236067No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT7152.252607038215557No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA6792.1391890614662423No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT6352.0005670898837464No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACCGGT6151.937557102800794No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT6101.9218046060300558No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT5301.6697646576982452No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT4741.4933366938659778No Hit
TTCGAGCATCAGTGTCAGTAACAGTCTAGTGAGCTGCCTTCGCAATCGGA4341.3673167197000724No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGAAAGCCGCCTTCGCCACTGGT4281.3484137235751865No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCTACTGGT2830.8915913172237799No Hit
TTCGAGCCTCAGCGTCAGTGACAGACCAGAGAGCCGCTTTCGCCACTGGT2200.6931098579124791No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT2170.6836583598500362No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACTGGT2120.6679058630792981No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT1480.4662739044138496No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT1290.40641441668504463No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT1120.35285592766453483No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT1080.3402539302479443No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT1040.32765193283135374No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT930.2929964399357298No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT800.2520399483318106No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT790.24888944897766296No Hit
TTCGCACCTGAGCGTCAGTCTTTGTCCAGGGGGCCGCCTTCGCCACCGGT720.22683595349862953No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT660.20793295737374373No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGACAGCCGCCTTCGCCACTGGT620.19533095995715322No Hit
TTCGCGCCTCACCGTCAGTTGCCGTCCAGTTATCCGCCTTCGCCACTGGT560.17642796383226741No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGCT560.17642796383226741No Hit
TTCGCTCCTCAGCGTCAGTAATAGCCCAGAGACCTGCCTTCGCCATTGGT550.17327746447811979No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT430.13547147222834818No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGGT410.12917047352005293No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCGCTGGT380.11971897545761004No Hit
TCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGAG360.11341797674931477No Hit
TCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGTG340.10711697804101951No Hit
TTCGAGCCTCAGCGTCAGTTACAAGCCAGAGAGCCGCTTTCGCCACCGGT320.10081597933272425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)