FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36909
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT562115.22934785553659No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT515213.95865507057899No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA388010.512341163401882No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT384710.42293207618738No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA34019.21455471565201No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT17294.684494296783982No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT15364.161586604893115No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACCGGT12113.2810425641442467No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT9052.4519764827006965No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT7301.9778373838359207No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT5391.4603484245035085No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA5351.4495109593865996No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCTAGTGAGCTGCCTTCGCAATCGGA4761.2896583489121893No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT4641.257145953561462No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG3640.986209325638733No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT3400.9211845349372783No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA3130.8480316453981414No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT2970.8046817849305048No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT2460.6665041046899131No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT2210.5987699477092309No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT2060.5581294535208216No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1550.4199517732802298No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT1260.34138015118263837No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT1120.30344902327345635No Hit
TTCGTGCTTCAGCGTCTGTTTCACCCCAGTATGCTGCCTTCGCAATCGGG980.26551789536427434No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT910.24655233140968325No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT910.24655233140968325No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT910.24655233140968325No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT900.243842965130456No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGAAAGCCGCCTTCGCCACTGGT850.23029613373431956No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGCAGGCTGCCTTCGCCATCGGT780.2113305697797285No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT720.1950743721043648No Hit
TTCGCGCCTCACCGTCAGTTACCGTCCAGTAATCCGCCTTCGCCACTGGT660.17881817442900105No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT590.15985261047441005No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA530.14359641279904628No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT510.13817768024059174No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG470.12734021512368257No Hit
TTCGCGCCTCAGCGTCAGTTGCGGCCCAGCAGGCTGCCTTCGCCATCGGT420.11379338372754612No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGGT410.11108401744831883No Hit
TTCGCACCTCAGCGTCAGTATTTGTCCAGCAAGCCGCCTTCGCCACTGGT410.11108401744831883No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT400.10837465116909156No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT400.10837465116909156No Hit
TCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGTG400.10837465116909156No Hit
TTCGTGCATGAGCGTCAGTCATGCCCCAGAGGGCTGCCTTCGCCATCGGT390.10566528488986425No Hit
TTCGAGCATCAGTGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA390.10566528488986425No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGCGT380.10295591861063697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)