FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31670
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT28028.847489737922324No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT22086.971897694979476No Hit
TTCGTGCCTCAGCGTCAGTTATGGCCCAGTAAACCGCCTTCGCCACTGGT13734.335333122829176No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT13484.25639406378276No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT11413.6027786548784335No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGG11143.5175244711083042No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT11063.492263972213451No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT10673.369119040101042No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT7542.3808020208399117No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT6622.0903062835490998No Hit
TTCGCATATGAGCGTCAGTATTTGCCCAGGAGACTGCCTTCGCCTTTGGT5751.815598358067572No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT5741.8124407957057151No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT5731.8092832333438587No Hit
TTCGTGCCTCAGCGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGAT5631.777707609725292No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT5501.7366592990211556No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT5471.7271866119355859No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT5331.6829807388695928No Hit
TTCGGGAATGAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT5061.5977265550994633No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG4231.3356488790653616No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT3751.1840858856962426No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT3581.1304073255446796No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT3521.1114619513735395No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT2890.9125355225765708No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT2680.8462267129775812No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT2510.7925481528260183No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGTAGGCCGCCTTCGCCACTGGT2300.7262393432270287No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGAAGGCTGCCTTCGCCATCGGT2030.6409851594568993No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT2010.634670034733186No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT1940.6125670982001894No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT1890.5967792863909062No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT1870.590464161667193No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT1820.5746763498579097No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA1780.5620461004104831No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1730.5462582886011998No Hit
TTCGAGCCTCAGCGTCATTCACCGTCCAGTAAGCCGCCTTCGCCACTGGT1570.49573729081149354No Hit
TTCGTGCTTCAGTGTCAGCATCCAGACCAGACGGCCGCCTTCGCCACCGG1550.4894221660877802No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT1550.4894221660877802No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT1540.48626460372592356No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1470.46416166719292706No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT1450.45784654246921375No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT1390.43890116829807385No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT1280.40416798231765083No Hit
TTCGTGCATCAGCGTCAGTAATGGCTTGGCAGGCTGCCTTCGCAATCGGG1260.39785285759393746No Hit
TTCGTGCCTCAACGTCAGTTACGGTCCAGAAAGTCGCCTTCGCCACTGGT1170.36943479633722764No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT1160.36627723397537104No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1140.3599621092516577No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA1100.34733185980423115No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC1080.34101673508051783No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA1070.33785917271866117No Hit
TTCGTGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACCGGT1050.33154404799494785No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT950.29996842437638144No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGAAAGCCGCCTTCGCCACTGGT900.2841806125670982No Hit
TTCGCCTATGAGCGTCAGTATTTGCCCAGGAGACTGCCTTCGCCTTTGGT890.28102305020524154No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT890.28102305020524154No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGCAGGCTGCCTTCGCCATCGGT850.26839280075781496No Hit
TTCGAGCATGAACGTCAGTTACGGATTAGTGTGCTGCCTTCGCAATCGGA830.26207767603410165No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT770.24313230186296178No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT760.23997473950110515No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA730.2305020524155352No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT710.22418692769182194No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT710.22418692769182194No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA710.22418692769182194No Hit
TTCGTGCTTCAGTGTCAGCAACAGACCAGACGGCCGCCTTCGCCACCGGT630.19892642879696873No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT630.19892642879696873No Hit
TTCGCGCCTCAGCGTCAGTGACGGCCCAGAGAGCTGCCTTCGCCATCGGT620.1957688664351121No Hit
TTCGCGCCTCAGCGTCAGTTGCAGTCCAGTTACCCGCCTTCGCCACTGGT620.1957688664351121No Hit
TTCGTGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACCGGT620.1957688664351121No Hit
TTCGAGCATCAGCGTCAGTTACAATCCAGTAAGCTGCCTTCGCAATCGGA610.19261130407325544No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT560.1768234922639722No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA560.1768234922639722No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA550.17366592990211557No Hit
TTCGCGCCTCACCGTCAGTTGCCGTCCAGTTATCCGCCTTCGCCACTGGT540.17050836754025891No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT530.16735080517840228No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT530.16735080517840228No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAGTCGCCTTCGCCACTGGT520.16419324281654563No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT520.16419324281654563No Hit
TTCGTGCCTCAGCGTCAGTTACGGATTCGCCAGATGCCTTCGCAATCGGT510.161035680454689No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT500.15787811809283234No Hit
TTCGCGCCTCAGCGTCGATGCCGGCCCAGGAGGCTGCCTTCGCCGTCGGT490.15472055573097568No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT480.15156299336911905No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT470.1484054310072624No Hit
TTCGTGTCTCAGCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT470.1484054310072624No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA460.14524786864540576No Hit
TTCGAGCCTCAACGTCAGTCACTGTCCAGTAAGCCGCCTTCGCCACTGGT450.1420903062835491No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCTCTGGT450.1420903062835491No Hit
TTCGAGTCTCAGCGTCAGTTATAGTCCAGCAAGCCGCCTTCGCCACCGGT440.13893274392169244No Hit
TTCGAGCCTCAACGTCAGTCACCGTCCAGTAAGCCGCCTTCGCCACTGGT440.13893274392169244No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT430.1357751815598358No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT430.1357751815598358No Hit
TTCGTGCCTCAGCGTCAGTTATGGCCCAGTAACCCGCCTTCGCCACTGGT420.13261761919797915No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT400.12630249447426586No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAATCGCCTTCGCCACTGGT400.12630249447426586No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT390.12314493211240923No Hit
TTCGGGAATTAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT390.12314493211240923No Hit
TTCGCATATTAGCGTCAGTATTTGCCCAGGAGACTGCCTTCGCCTTTGGT380.11998736975055257No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT370.11682980738869592No Hit
CTCGCGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT350.11051468266498263No Hit
TTCGTACCTCAGTGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT350.11051468266498263No Hit
TTCGAGCCTCAGCGTCAGTGACAGACCAGAGAGCCGCTTTCGCCACCGGT340.107357120303126No Hit
TTCGTGCCTCAGCGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGCT340.107357120303126No Hit
TTCGTACCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT340.107357120303126No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT340.107357120303126No Hit
TTCGTGCATGAGCGTCAGTATTATCCCAGGAGGCTGCCTTCGCCATCGGT330.10419955794126934No Hit
TTCGAGCATGAACGTCAGTTACAGTTTAGCAAGCTGCCTTCGCAATCGGG330.10419955794126934No Hit
TTCGCACATGAGCGTCAGTTGTGTGCCAGGAAGCCGCCTTCGCCACTGGT330.10419955794126934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)