FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31670
Sequences flagged as poor quality0
Sequence length200
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA24327.679191664035365No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA23907.546574044837386No Hit
TCCGGATTTATTGGGCGTAAAGGGTGCGTAGGCGGTTTGTTAAGTATGAG13934.398484370066309No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGTGGCAGATCAAGTCTGGA13304.19955794126934No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT11343.580675718345437No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGTGATCAAGTCAGCT9993.15440479949479No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT7052.226081465108936No Hit
ATCGGAATAACTGGGCGTAAAGGGCATGCAGGCGGTTCATCAAGTAGGAT6362.0082096621408274No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAAGGCAAGTCAGAT6281.9829491632459741No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT6171.9482159772655512No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG6161.9450584149036942No Hit
TCCGGAATGACTGGGCGTAAAGGGTGTGTAGGCGGGCTCGCAAGTTGGAT5901.8629617934954217No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA5681.7934954215345755No Hit
TCCGGAATCATTGGGCGTAAAGAGGGAGCAGGCGGCCGCAAGGGTCTGTG5651.7840227344490054No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCGATTAAGTTAGAT5411.7082412377644458No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGATAGCAAGTCAGCT5251.6577202399747395No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTAGCTTAAGTTGGAT5071.6008841174613198No Hit
TCCGGAATTACTGGGTGTAAAGGGAGTGTAGGCGGGATATCAAGTCAGAA4651.4682664982633407No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG4451.4051152510262077No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT3831.2093463845910957No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA3641.1493526997158194No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT3641.1493526997158194No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAATACAAGTTAGTT3471.0956741395642564No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA3451.089359014840543No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGACGGTAAGTCAGGT3030.9567413956425639No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT2770.8746447742342912No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAA2360.7451847173981686No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA2300.7262393432270287No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGCAAGTCAGAA2050.6473002841806126No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT1890.5967792863909062No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG1860.5873065993053362No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT1830.5778339122197663No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGTCAAGCGGGGT1810.571518787496053No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT1740.5494158509630565No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT1620.5115251026207768No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGAGGCAAGTTGAAT1590.5020524155352069No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT1570.49573729081149354No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGGGATCAAGTCAGCT1360.429428481212504No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT1330.41995579412693396No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGG1300.4104831070413641No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGCAGCAAGTCTGAT1250.3946952952320808No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAGACAAGTTGGTT1250.3946952952320808No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG1220.3852226081465109No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA1210.3820650457846542No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG1180.3725923586990843No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCCATGCAAGTCAGAA1110.35048942216608775No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT1090.3441742974423745No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT1080.34101673508051783No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT1050.33154404799494785No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG980.30944111146195136No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTAAGACAAGTCAGAA950.29996842437638144No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGGAAGCAAGTCAGAT940.2968108620145248No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT940.2968108620145248No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCGTTAAGTCAGCT890.28102305020524154No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGCAATTTAAGTTGGAT870.2747079254815282No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGTTAAGTCTGAA850.26839280075781496No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGAT810.2557625513103884No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT740.23365961477739183No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCGCCTAAGCGGAAC720.22734449005367857No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT680.214714240606252No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGTGGCAGAACAAGTCTGGA660.20839911588253868No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA630.19892642879696873No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAA620.1957688664351121No Hit
TCCGGAATGATTGGGCGTAAAGGGCGTGTAGGCGGCTTTATAAGTCTGGA610.19261130407325544No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTT610.19261130407325544No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT610.19261130407325544No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT600.1894537417113988No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGAT600.1894537417113988No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA550.17366592990211557No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGCGGCAGTACAAGTCAGGA550.17366592990211557No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA530.16735080517840228No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGTTGTTAAGTCAGTT530.16735080517840228No Hit
TCCGGATTCATTGGGTTTAAAGGGTGCGTAGGCTGTGCGTCAAGTCAGGG520.16419324281654563No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT510.161035680454689No Hit
CTCGGAATAACTGGGCGTAAAGGGCATGCAGGCGGTTCATCAAGTAGGAT500.15787811809283234No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTAATTAAGTTGGAT480.15156299336911905No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGCGGCCATGCAAGTCAGAA470.1484054310072624No Hit
TCCGGAATGATTGGGCGTAAAGCGCGCGCAGGCGGCCGCTCAAGCGGGAC470.1484054310072624No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCT470.1484054310072624No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTAGCAAGTCTGAA460.14524786864540576No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAATTAAGTTAGTT450.1420903062835491No Hit
TCCGGAATTATTGGGCGTAAAGAGTACGTAGGCGGTTTGCTAAGCGCAAG450.1420903062835491No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT440.13893274392169244No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCTGCAAGTCTGAA440.13893274392169244No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA440.13893274392169244No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTCAGAT430.1357751815598358No Hit
TCCGGAATCATTGGGCGTAAAGCGCGCGCAGGCGGGCTTTCAAGCGGCGG420.13261761919797915No Hit
TCCGGATTTACTGGGTGTAAAGGGTGAGTAGGCGGGAAGTCAAGTCAGAT420.13261761919797915No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGAGACAAGTTGAAT410.12946005683612252No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA410.12946005683612252No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATAGGCAAGTCTGGA410.12946005683612252No Hit
TCCGGAATGACTGGGCGTAAAGGGAGCGTAGGCGGCACAACAAGTTAGGA400.12630249447426586No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGAAGGCAAGTTGGAT390.12314493211240923No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCCGGCAAGTCAGAT390.12314493211240923No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGGATGGCAAGTCAGAT380.11998736975055257No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT370.11682980738869592No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGTCGGTAAGTTGGGA370.11682980738869592No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGCTGCAAGTCAGAT350.11051468266498263No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT340.107357120303126No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGAAAAGTCTGAA330.10419955794126934No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGAGATTAAGTCAGTT320.10104199557941271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)