FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37393
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT27017.2232770839461935No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT21845.8406653651753No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT19315.164068141095926No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT18064.829780975048806No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGAT16894.5168881876287No Hit
TTCGTGCCTCAGTGTCAGTTGCTGTCCAGATAGTCGCCTTCGCCACTGAT8642.310592891717701No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT7702.0592089428502662No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT7321.957585644371941No Hit
TTCGAGCATGAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGG7211.9281683737597946No Hit
TTCGAGCATCAATGTCAGTTACAGCTTGGTGCACTGCCTTCGCAATCGGA6021.609926991682935No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA5711.5270237745032493No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT5181.38528601609927No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT4911.3130799882330917No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT4841.294359906934453No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT4721.2622683389939293No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT4391.1740165271574894No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT4241.133902067231835No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT4211.125879175246704No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT4211.125879175246704No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT4111.0991362019629343No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT4001.0697189313507875No Hit
TTCGTGCATGAGCGTCAGTTTTGCCCCAGGGGGCTGCCTTCGCCATCGGT3911.0456502553953948No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT3660.9787928221859705No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA3600.9627470382157088No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT3550.949375551573824No Hit
TTCGTGCTTCAGTGTCAGCAACAGACCAGACGGCCGCCTTCGCCACCGGT2870.7675233332441901No Hit
TTCGCACCTCAGCGTCAATACCGGTCCAGGTGGCCGCCTTCGCCACTGAT2870.7675233332441901No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT2680.7167116840050277No Hit
TTCGAGCCTCAACGTCAGTGGCGGTTTAGCAGGCTGCCTTCGCAATCGGG2640.7060144946915198No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT2370.6338084668253416No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT2280.6097397908699489No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA2210.5910197095713101No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT2180.5829968175861793No Hit
TTCGAGCATCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCACTGGT2130.5696253309442944No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG2120.5669510336159175No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT2100.5616024389591635No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT2070.5535795469740326No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT2030.5428823576605247No Hit
TTCGTGCCTCAGCGTCAGTTGAGCGCCGGTAAGCTGCCTTCGCAATCGGC1990.5321851683470168No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT1880.5027678977348702No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCTCTGGT1770.4733506271227235No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT1710.45730484315246167No Hit
TTCGTTCCTCAGTGTCAATGTCGGCCCAGTAAAGCGCTTACGCCACCGAT1660.44393335651057686No Hit
TTCGTGCCTGAGCGTCAGTTGAGCGCCGGTAAGCTGCCTTCGCAATCGGC1650.4412590591821999No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT1640.43858476185382295No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACCGGT1580.4225389778835611No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1560.4171903832268071No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT1500.40114459925654533No Hit
TTCGTCTTTCAGAGTCAGTTTCCGTCCAGCAAGCTGCCTTCGCTATTGGT1450.3877731126146605No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA1440.38509881528628354No Hit
TTCGTGCATCAGCGTCAATCGCGGATTCGTCAGATGCCTTCGCAATCGGT1370.36637873398764476No Hit
TTCGAGCCTCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA1310.35033295001738296No Hit
TTCGTCTCTCAGAGTCAGTTACTGTCCAGCAAGCTGCCTTCGCTCTTGGT1300.347658652689006No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT1290.344984355360629No Hit
TTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAGCCGCCTTCGCCACTGGT1240.3316128687187441No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT1240.3316128687187441No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT1230.3289385713903672No Hit
TTCGAGCATGAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGA1180.31556708474848233No Hit
TTCGTTCCTCAGCGTCAATTACGGCCCAGTAAAGCGCTTACGCCACCGAT1180.31556708474848233No Hit
TTCGTGCTTCAGCGTCAGTCGGGCGCCGGTACGCTGCCTTCGCAATCGGA1170.31289278742010534No Hit
TTCGAGCCTCAGCGTCAGTTGCACCCCGGATACCTGCCTTCGCGATCGGA1170.31289278742010534No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGCAAGCTGCCTTCGCAATCGGA1140.30486989543497445No Hit
TTCGTGCCTCAGCGTCAGACAGAAGCCGGCAGGCTGCCTTCGCAATCGGA1130.30219559810659746No Hit
TTCGTGCCTCAGCGTCAGTCGGGCGCCGGTATGCTGCCTTCGCAATCGGA1130.30219559810659746No Hit
TTCGTGCATCAGCGTCAATTCTAGACCAGTAAGCCGCCTTCGCCACTGGT1130.30219559810659746No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT1120.2995213007782205No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1090.29149840879308964No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAGTCGCCTTCGCCACTGGT1070.28614981413633567No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA1060.28347551680795874No Hit
TTCGAGCCTCAATGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACCGGT1050.28080121947958175No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC1030.2754526248228278No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1010.27010403016607387No Hit
TTCGAGCCTCAACGTCAGTCGCGGCTTAGCAGGCTGCCTTCGCAATCGGG1000.2674297328376969No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1000.2674297328376969No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT980.2620811381809429No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT950.2540582461958121No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT950.2540582461958121No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT860.2299895702404193No Hit
TTCGTGCCTCAACGTCAGTTATTGTCCAGAAAGTCGCCTTCGCCACCGGT860.2299895702404193No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT850.22731527291204234No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA830.22196667825528843No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT790.21126948894178055No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT770.2059208942850266No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT770.2059208942850266No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT760.20324659695664962No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGG750.20057229962827267No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGCT740.19789800229989568No Hit
TTCGTGCCTCAATGTCAGTTACAGTCCAGAAAGTCGCCTTCGCCACTGGT720.19254940764314177No Hit
TTCGTTCCTCAGTGTCAATTACGGCCCAGTAAAGCGCTTACGCCACCGAT710.18987511031476478No Hit
TTCGAGCCTCAACGTCAGTGGCGGTTTAGCAGGCTGCCTTCGCCATCGGG690.18452651565801084No Hit
TTCGTGTCTCAGCGTCAGTTGTGGTCCAGTAAACCGCCTTCGCCACCGGT680.18185221832963389No Hit
TTCGAGCATCAGCGTCAGTGGTGCTGCGGCATGCTGCCTTCGCAATCGGA680.18185221832963389No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGAAAGCCGCCTTCGCCACTGGT660.17650362367287994No Hit
TTCGTGCTTCAGCGTCAGTTATAGTATGGTATGCTGCCTTCGCAATCGGA660.17650362367287994No Hit
TTCGCGCCTCAACGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT650.173829326344503No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT650.173829326344503No Hit
TTCGTGCCTCAGCGTCAATAAATGGCCAGATACCCGCCTTCGCCACTGGT640.171155029016126No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT630.16848073168774905No Hit
TTCGAGCATTAACGTCAGTTACGGCTTAGTGTGCTGCCTTCGCAATCGGG610.1631321370309951No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAGTCGCCTTCGCCACTGGT610.1631321370309951No Hit
TTCGTGCCTGAGCGTCAGTTATGTCCCAGGAGGCTGCCTTCGCCATCGGT600.16045783970261815No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT570.15243494771748722No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGCAAGCCGCCTTCGCCACTGGT560.14976065038911024No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG550.1470863530607333No Hit
TTCGTGCTTCAGTGTCAGTTGCAGACCAGGCGGCCGCCTTCGCCACTGGT540.14441205573235633No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT530.14173775840397937No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA510.1363891637472254No Hit
CTCGCGCATCAGCGTCAGATCGAGCCTGGGAAGCTGCCTTCGCTATCGGG500.13371486641884844No Hit
TTCGTGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACCGGT490.13104056909047146No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT480.12836627176209453No Hit
TTCGCATCTCAGCGTCAGCCACAGTCCAGAAAGGCGCCTTCGCCACTGGT470.12569197443371755No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT470.12569197443371755No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT450.1203433797769636No Hit
TTCGTGCCTCAGCGTCAATGGCGGATTCGTGAGATGCCTTCGCAATCGGT440.11766908244858663No Hit
TTCGTGCCTCAGCGTCAGTTGACAGCCAGATACTCGCCTTCGCCACTGGT430.11499478512020965No Hit
TTCGCGCCTCACCGTCAGTTACCGTCCAGTAATCCGCCTTCGCCACTGGT430.11499478512020965No Hit
TTCGTACCTCAGTGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT430.11499478512020965No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT420.11232048779183271No Hit
TTCGCACATGAGCGTCAGTAGTGTGCCAGGAAGCCGCCTTCGCCACTGGT410.10964619046345574No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGAAAGCCGCCTTCGCCACTGGT410.10964619046345574No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT400.10697189313507877No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT380.10162329847832481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)