FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37393
Sequences flagged as poor quality0
Sequence length200
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTTTGCAAGTCTGAA19785.289760115529645No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT19375.180113925066189No Hit
TCCGGAATAATTGGGCGTAAAGGGCGCGTAGGCGGCTCGGTAAGTCTGGA18705.000936004064932No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCTTAAGTCAGGT17804.760249244511005No Hit
TCCGGAATGACTGGGCGTAAAGGGTGTGTAGGTGGTTTGATAAGTTAGAT8862.369427432941994No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGAGATTAAGTCAGCT8432.2544326478217847No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT8302.2196667825528844No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA7281.9468884550584333No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGACGGGTCGTTAAGTCAGCT6931.8532880485652394No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGCTTTTAAGTCAGTT6581.7596876420720455No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTTGGGA6291.6821330195491135No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT6041.6152755863396893No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT5141.374588826785762No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGATAGCAAGTCAGCT4581.2248281763966518No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA4501.203433797769636No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTCTTTTAAGTCAGCG4461.1927366084561282No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT4301.1499478512020966No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA4101.0964619046345574No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAGACCAAGTCTGAA3951.0563474447089027No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCCTATTAAGTCGGGC3931.0509988500521488No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGTGGCAGAACAAGTCTGGA3520.9413526595886931No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT3500.9360040649319392No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCGATTAAGTTAGAT3430.9172839836333003No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT3350.8958896050062846No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG3190.8531008477522531No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGAT3000.8022891985130907No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCTGTTATGTAAGTCAGGG2920.7808948198860749No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTCTTTTAAGTTGGAT2910.7782205225576979No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCTGATAAGTTGGAT2640.7060144946915198No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG2630.7033401973631428No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA2610.6979916027063889No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGAGACAAGTCTGAA2540.67927152140775No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT2420.6471799534672265No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT2200.5883454122429332No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTAGCTTAAGTTGGAT2130.5696253309442944No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT2120.5669510336159175No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT2100.5616024389591635No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT2100.5616024389591635No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCACTGCAAGTCTGAA2030.5428823576605247No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA2010.5375337630037708No Hit
TCCGGAATGACTGGGCGTAAAGGGAGCGTAGGCGGCTTGGCAAGTTAGGA1980.5295108710186398No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGAGCGTCAAGTCAGCG1950.521487979033509No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG1890.5054421950632471No Hit
TCCGGAATCATTGGGCGTAAAGAGTTCGTAGGTGGCATGTAAAGTCCGGT1840.4920707084213623No Hit
TCCGGAATGACTGGGCGTAAAGGGAGCGTAGGCGGCACAACAAGTTAGGA1730.46265343780921564No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTTATAAGTTAGAG1680.44928195116733083No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA1680.44928195116733083No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA1660.44393335651057686No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGCAGGCGGTACGGAAAGTCAGCG1620.433236167197069No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGTCGGCAAGTCAGAT1550.41451608589843025No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT1540.4118417885700532No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT1520.40649319391329924No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGTGCAAGTCAGAA1510.4038188965849223No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT1500.40114459925654533No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGCTGCCAAGTCAGCG1500.40114459925654533No Hit
TTCGGATTTATTGGGCGTAAAGGGTCTGTAGGAGGTTCGTTAAATACGAG1460.39044740994303745No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTGTTAAGTCAGCG1460.39044740994303745No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCT1390.37172732864439867No Hit
TCCGGAATCATTGGGCGTAAAGAGTTCGTAGGCGGCATGTAAAGTCAGGT1350.36103013933089084No Hit
TTCGGATTTATTGGGCGTAAAGGGTCTGTAGGAGGTTTGTTAAATTCGAG1350.36103013933089084No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGAT1350.36103013933089084No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACAGCAAGTCTGAT1350.36103013933089084No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGATTTCTAAGTCAGCG1350.36103013933089084No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGATGGCTAAGTCTGAT1290.344984355360629No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA1280.342310058032252No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCTGAA1240.3316128687187441No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT1220.3262642740619902No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGATGGCATGGCAAGTCTGAA1200.3209156794052363No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT1180.31556708474848233No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCGGACTCTTAAGTCAGTT1150.30754419276335143No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT1120.2995213007782205No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGAGAAGCAAGTCAGTG1110.29684700344984355No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA1070.28614981413633567No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG1060.28347551680795874No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG1030.2754526248228278No Hit
TCCGGAATCATTGGGCGTAAAGGGCGCGTAGGCGGCTCGGTAAGTCTGGA1030.2754526248228278No Hit
TCCGGAATTATTGGGCGTAAAGCGTGCGTAGGCGGCCACTTAAGTCTGTC1000.2674297328376969No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT930.2487096515390581No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAATACAAGTTAGTT900.2406867595539272No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT900.2406867595539272No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA890.23801246222555023No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATTGCAAGTCAGAT840.22464097558366541No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA810.2166180835985345No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT810.2166180835985345No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT800.21394378627015753No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT800.21394378627015753No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTCTGGCAAGTCTGAT790.21126948894178055No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA770.2059208942850266No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCGTTAAGTCAGCT750.20057229962827267No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGAATGCAAGTCAGAT740.19789800229989568No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTCCGGAAAGTCAGCG680.18185221832963389No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGTCTCAAAAGCGTGTT660.17650362367287994No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATAACAAGTCTGAT660.17650362367287994No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGGTAAGTCAGCG650.173829326344503No Hit
TCCGGAATTACTGGGTGTAAAGGGCGTGTAGGCGGGTAATCAAGTCAGAT640.171155029016126No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGACTGTCAAGTCAGTC630.16848073168774905No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGTGATTAAGTCAGCG620.16580643435937206No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA610.1631321370309951No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGATGGCTAAGCAAGTTAGTC590.15778354237424116No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGTTATGTAAGATAGCG590.15778354237424116No Hit
TCCGGAATGACTGGGCGTAAAGGGCGTGTAGGCGGCAGTATAAGTCCGGA590.15778354237424116No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA580.1551092450458642No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG570.15243494771748722No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAA560.14976065038911024No Hit
TCCGGAATCATTGGGCGTAAAGAGTTCGTAGGTGGTTTGTTAAGTTTGGT540.14441205573235633No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGCTGCAAGTCAGAT530.14173775840397937No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGGCTTTTAAGTCTGAC520.13906346107560238No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGACAACAAGTTAGTT510.1363891637472254No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGTCAGAT500.13371486641884844No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT500.13371486641884844No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCGATGCAAGTCTGGA490.13104056909047146No Hit
TCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCACGATAAGTCTGTA470.12569197443371755No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG470.12569197443371755No Hit
TCCGGAATGACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTCAGAT450.1203433797769636No Hit
TCCGGAATCACTGGGCGTAAAGGGCGCGTAGGTGGTCTGTTAAGTCAGAT450.1203433797769636No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTAAGCAAGTCAGAA450.1203433797769636No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAAGCAAGTTAGTT450.1203433797769636No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGGATCTTAAGTCAGGT430.11499478512020965No Hit
TTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTCGTTAAGTCTGCT430.11499478512020965No Hit
ATCGGAATTACTGGGCGTAAAGGGTGCGCAGGCGGTTGAGTAAGACAGAT420.11232048779183271No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT420.11232048779183271No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAGATAAGTTAGTT410.10964619046345574No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT400.10697189313507877No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGATTTGTAAGATAGTG400.10697189313507877No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGCAATTTAAGTTGGAT390.10429759580670178No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGCATGCAAGTCAGAT380.10162329847832481No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT380.10162329847832481No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGTATCACAAGTCAGAA380.10162329847832481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)