FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37500
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT382910.210666666666667No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT26166.976No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT19515.2026666666666666No Hit
TTCGGGAATGAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT15564.149333333333334No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT12083.221333333333333No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT10322.752No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT10012.6693333333333333No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT7331.9546666666666668No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT7311.9493333333333336No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT6661.7760000000000002No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT6101.6266666666666665No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT6081.6213333333333333No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT5771.5386666666666666No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT5411.4426666666666668No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT5091.3573333333333333No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT4951.32No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT4191.1173333333333333No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT4031.0746666666666667No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT4021.072No Hit
TTCGGGAATGAGCGTCAGTTGCGTCCCAGACCGTCGCCTTCGCCACTGGT3781.008No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT3650.9733333333333334No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT3550.9466666666666667No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAGTCGCCTTCGCCACTGGT3460.9226666666666666No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT3460.9226666666666666No Hit
TTCGAGCCTCAGCGTCATTCACCGTCCAGTAAGCCGCCTTCGCCACTGGT3440.9173333333333333No Hit
TTCGTGCTTCAGCGTCTGTCCTGGTCTGGTATGCTGCCTTCGCAATCGGG3430.9146666666666667No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT3380.9013333333333333No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA3380.9013333333333333No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT3150.84No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT2910.776No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT2780.7413333333333333No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC2770.7386666666666666No Hit
TTCGTGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACCGGT2770.7386666666666666No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT2700.72No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT2690.7173333333333334No Hit
TTCGTGCCTCAGCGTCAGTTACAGCCCAGCAAATCGCCTTCGCCACTGGT2570.6853333333333333No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT2530.6746666666666666No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT2520.672No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCTCTGGT2380.6346666666666666No Hit
TTCGTGCATCAGCGTCAATTCTAGACCAGTAAGCCGCCTTCGCCACTGGT2010.536No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA1930.5146666666666666No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCACTGGT1820.48533333333333334No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT1810.4826666666666667No Hit
TTCGCTCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1760.4693333333333333No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT1700.45333333333333337No Hit
TTCGCATCTGAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACCGGT1560.416No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT1440.384No Hit
TTCGTGCTTCAGTGTCAGTTATAGTTTAGTAAGCTGCCTTCGCAATCGGA1390.37066666666666664No Hit
TTCGTGCCTCAACGTCAGTTATTGTCCAGAAAGTCGCCTTCGCCACCGGT1370.36533333333333334No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1360.36266666666666664No Hit
TTCGCACATGAGCGTCAGTTGTGTGCCAGGAAGCCGCCTTCGCCACTGGT1350.36No Hit
TTCGGGAATTAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT1330.3546666666666667No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT1320.35200000000000004No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA1310.34933333333333333No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT1290.344No Hit
TTCGTGCCTCAGCGTCAGTTATGGCCCAGTAAACCGCCTTCGCCACTGGT1260.336No Hit
TTCGTGCCTCAGCGTCAGTAACGGCCCAGCAAACCGCTTTCGCCACTGGT1210.32266666666666666No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT1150.3066666666666667No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1130.30133333333333334No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT1070.2853333333333333No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT1050.27999999999999997No Hit
TTCGTACCTCAGTGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT1000.26666666666666666No Hit
TTCGAGCCTCAGTGTCAGTTAGAGCCCAGCAGGCCGCCTTCGCCACTGGT990.264No Hit
TTCGTGCCTCAGCGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGAT890.2373333333333333No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT890.2373333333333333No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATTGGA860.22933333333333333No Hit
TTCGTGCCTCAGTGTCAGTTACAGACCAGGCGACCGCCTTCGCCACTGGT840.22399999999999998No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT790.2106666666666667No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA770.2053333333333333No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGTCGCCTTCGCCACTGGT760.20266666666666666No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGAAAGCCGCCTTCGCCACTGGT760.20266666666666666No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGAGAGCCGCCTTCGCAACTGGT740.19733333333333333No Hit
TTCGTGCCTCAGCGTCAGTGATGGCCCAGCAAGTCGCCTTCGCCACTGGT730.19466666666666665No Hit
TTCGAGCCTCAGCGTCAGTTGATGCCCAGTAGGCCGCCTTCGCCACTGGT710.18933333333333333No Hit
TTCGTACCTCAGCGTCAGTTACAGCCCAGCAAATCGCCTTCGCCACTGGT700.18666666666666668No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACCGGT680.18133333333333332No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT660.17600000000000002No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA660.17600000000000002No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT650.17333333333333334No Hit
TTCGGGCATGAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT630.168No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT570.152No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT560.14933333333333332No Hit
TTCGTGCTTCAGTGTCAGTTGCAGGCCAGGCGACCGCCTTCGCCACCGGT550.14666666666666667No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT540.14400000000000002No Hit
TTCGTGTCTCAGCGTCAGTAATGGCCCAGCAAGCCGCCTTCGCCACTGGT540.14400000000000002No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT530.14133333333333334No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT520.13866666666666666No Hit
TTCGCGCCTCAGCGTCAGTTTCGGCCCAGAGAGCTGCCTTCGCCGTCGGT520.13866666666666666No Hit
TTCGTGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT500.13333333333333333No Hit
TTCGTGTCTCAGCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT500.13333333333333333No Hit
TTCGCGCCTCAACGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT490.13066666666666668No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT480.128No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT470.12533333333333332No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT470.12533333333333332No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT450.12No Hit
TTCGTGCCTCAGCGTCAGTTTCAGTCCAGTTAACCGCCTTCGCCACTGGT450.12No Hit
TTCGTTACTCACCGTCAAGATCGTTCCAGTTAGACGCCTTCGCCACAGGT440.11733333333333333No Hit
TTCGTGTCTCAGCGTCAGTAACAGCCCAGCAAATCGCCTTCGCCACTGAT430.11466666666666667No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT420.11199999999999999No Hit
TTCGTGATTCAGCGTCAGTTGTGGTCCAGTAATCCGCCTTCGCCACTGAT410.10933333333333334No Hit
TTCGTGCTTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT410.10933333333333334No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT410.10933333333333334No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT400.10666666666666667No Hit
TTCGTGCCTCAACGTCAGTTACGGTCCAGAAAGTCGCCTTCGCCACTGGT390.104No Hit
TTCGCCCCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT380.10133333333333333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)