FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48698
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT630212.940983202595588No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT604912.42145467986365No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA507810.427532958232371No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA31756.519774939422564No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT25995.336974824428108No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA24875.1069859131791855No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT12702.6079099757690254No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT8101.6633126617109533No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA7901.6222432132736455No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT6941.4251098607745698No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT6851.4066286089777815No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA6651.365559160540474No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT6571.349131381165551No Hit
TTCGTGTCTCAGCGTCAGTATTGGCCTAGTGAGCCGCCTTCGCCACTGGT6541.3429709638999547No Hit
TTCGTTCCTCAGTGTCAATTACGGCCCAGTAAAGCGCTTACGCCACCGAT6421.3183292948375704No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT6251.283420263665859No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT4750.9753994003860529No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT4680.9610250934329952No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT4460.9158487001519569No Hit
TTCGTGCCTCAGCGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGAT4130.8480841102303996No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT3960.8131750790586882No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT3600.7392500718715347No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG3600.7392500718715347No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG3120.6406833956219967No Hit
TTCGTGCCTGAGCGTCAGTTATGTCCCAGGAGGCTGCCTTCGCCATCGGT3120.6406833956219967No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT2920.5996139471846893No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCTCTGGT2840.5831861678097663No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT2790.5729188057004395No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT2580.5297958848412666No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT2540.5215819951538051No Hit
TTCGAGCCTCAGCGTCATTCACCGTCCAGTAAGCCGCCTTCGCCACTGGT2200.4517639328103823No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT2140.43944309827919015No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1920.39426670499815186No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG1900.3901597601544211No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA1760.3614111462483059No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT1640.3367694771859214No Hit
TTCGTACCTCAGCGTCAGTAATGGCCCAGCAAGTCGCCTTCGCCACTGGT1630.33471600476405605No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT1370.28132572179555626No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1370.28132572179555626No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT1280.2628444699987679No Hit
TTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAGCCGCCTTCGCCACTGGT1240.2546305803113064No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT1240.2546305803113064No Hit
TTCGTGTCTCAGCGTCAGGTATCGTCCAGTAACTCGCCTTCGCCACTGGT1180.24230974578011416No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACCGGT1160.23820280093638344No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT1050.2156146042958643No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT1040.21356113187399892No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT1040.21356113187399892No Hit
TTCGTGCCTCAACGTCAGTTTCAGTCCAGTAAGTCGCCTTCGCCACTGGT1030.21150765945213354No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT1030.21150765945213354No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT1030.21150765945213354No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT1010.20740071460840281No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGCAAGCCGCCTTCGCCACTGGT1000.20534724218653744No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA980.20124029734280668No Hit
TTCGTGCCTCAGCGTCAATCAATGCCCAGATAGCCGCCTTCGCCTCTGGT980.20124029734280668No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT930.1909729352334798No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT920.18891946281161445No Hit
CTCGCGCATCAGCGTCAGATCGAGCCTGGGAAGCTGCCTTCGCTATCGGG920.18891946281161445No Hit
TTCGTGTCTCAGCGTCAGTAATGGCCCAGCAAGCCGCCTTCGCCACTGGT920.18891946281161445No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT910.18686599038974908No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT900.18481251796788367No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT880.18070557312415295No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT870.17865210070228757No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT790.16222432132736456No Hit
TTCGAGCCTCAACGTCAGTCACTGTCCAGTAAGCCGCCTTCGCCACTGGT730.14990348679617232No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA700.14374306953057622No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT700.14374306953057622No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGGT700.14374306953057622No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTTGCCACTGGT690.14168959710871085No Hit
TTCGTGCCTCAGCGTCAGTTAGAGCCCAGCAGATCGCCTTCGCCACTGGT620.1273152901556532No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT600.12320834531192246No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT580.11910140046819172No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT570.11704792804632634No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT550.11294098320259557No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG520.10678056593699946No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT520.10678056593699946No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT490.10062014867140334No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT490.10062014867140334No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGCT490.10062014867140334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)