FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108291
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGTGCCTCAGCGTCAGTCGGGCGCCGGTACGCTGCCTTCGCAATCGGA97969.045996435530192No Hit
TTCGTGCCTGAGCGTCAGTAAGGCGCCGGTATGCTGCCTTCGCAATCGGA72516.6958473003296675No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA72086.656139476041408No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT57115.27375312814546No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT53714.959784284935959No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT35213.2514244027666193No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT30952.8580399109805987No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGTCGCCTTCGCCACCGGT24172.231949100109889No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT23572.176542833661154No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT20631.9050521280623505No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA19141.7674598997146578No Hit
TTCGTGCCTCAGCGTCAATTGCGGATTCGTGAGATGCCTTCGCAATCGGT17691.633561422463547No Hit
TTCGTGCCTCAGCGTCAGTTATCGTCCAGTAAGTCGCCTTCGCCACTGAT17661.6307911091411105No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT16071.483964503051962No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT16061.483041065277816No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT16021.4793473141812339No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA15211.4045488544754412No Hit
TTCGTGTCTCAGCGTCAGGTATCGTCCAGTAACTCGCCTTCGCCACTGGT13861.2798847549657866No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT12871.1884644153253732No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT11941.1025847023298336No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA11801.0896565734917953No Hit
TTCGCACCTCAGCGTCAATACCGGTCCAGGTGGCCGCCTTCGCCACTGAT11071.0222456159791673No Hit
TTCGAGCCTCAGCGTCAGTTGCACTCCGGACATATGCCTTCGCGATCGGA10730.9908487316582173No Hit
TTCGTGCCTCAGCGTCAGTAACGGCCCAGCAAACCGCTTTCGCCACTGGT10640.9825377916909069No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT10560.9751502894977423No Hit
TTCGAGCTTCAGCGTCAGTTGCGCTCCAGTGAGCTGCCTTCGCAATCGGA9200.8495627522139421No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA9200.8495627522139421No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT8390.7747642925081493No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT8190.7562955370252376No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT8180.7553720992510919No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT7990.7378267815423257No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA7680.7092002105438125No Hit
TTCGTGTCTCAGCGTCAGTAATGGTCCAGTAAACCGCCTTCGCCACTGGT7180.663028321836533No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT7130.6584111329658051No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG6680.6168564331292535No Hit
TTCGCGCCTCACCGTCAGTTGCTGTCCAGCAATCCGCCTTCGCCACTGGT6580.6076220553877977No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA6560.6057751798395066No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT6410.5919236132273227No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA5900.5448282867458977No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT5890.5439048489717521No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG5790.5346704712302961No Hit
TTCGTGCATCAGCGTCAGTAATGGCTTGGCAGGCTGCCTTCGCAATCGGG5580.5152782779732388No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGTAGACCGCCTTCGCCACTGGT5540.5115845268766565No Hit
TTCGTGCCTTAGCGTCAGTAAGGCGCCGGTATGCTGCCTTCGCAATCGGA5500.507890775780074No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT5450.5032735869093461No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT5090.47002982704010493No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT4820.445097007138174No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT4550.42016418723624305No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT4270.3943079295601666No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT4120.38045636294798274No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT4020.3712219852065269No Hit
TTCGTGCTTCAGCGTCAGCGGTAGTGTAGTAAGCTGCCTTCGCAATCGGG3460.3195094698543739No Hit
TTCGTCTCTCAGAGTCAGTTACTGTCCAGCAAGCTGCCTTCGCTCTTGGT3450.3185860320802283No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC3430.3167391565319371No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT3350.3093516543387724No Hit
TTCGTACCTCAGCGTCAGTTACAGCCCAGCAAATCGCCTTCGCCACTGGT3220.29734696327487975No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT3140.289959461081715No Hit
TTCGCGCCTCAGTGTCAGTCTATGTCCAGATAGCCGCCTTCGCCACTGGT2650.24471101014858113No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT2530.23362975685883408No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT2370.21885475247250463No Hit
TTCGTGCCTCAGTGTCAGTTACGGCCCAGTATGTCGCTTTCGCCACCGGT2290.2114672502793399No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT2260.20869693695690317No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA2170.20038599698959286No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT2030.1874578681515546No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT2000.18468755482911783No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT2000.18468755482911783No Hit
TTCGTGCCTGAGCGTCAGTTATGTCCCAGGAGGCTGCCTTCGCCATCGGT1920.17730005263595314No Hit
CTCGCGCATCAGCGTCAGATCGAGCCTGGGAAGCTGCCTTCGCTATCGGG1910.17637661486180756No Hit
TTCGTGTCTCAGCGTCAGTAATGGCCCAGCAAATCGCCTTCGCCACTGGT1880.17360630153937076No Hit
TTCGTGTCTCAGCGTCAGTTGTGGTCCAGTAAACCGCCTTCGCCACCGGT1830.16898911266864283No Hit
TTCGAGCATCAGTGTCAGTAACAGTCTAGTGAGCTGCCTTCGCAATCGGA1740.16067817270133253No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT1710.15790785937889576No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT1690.15606098383060457No Hit
TTCGAGCTTCAGCGTCAGTTACGCTCCCGCCAGCTGCCTTCGCAATCGGG1660.1532906705081678No Hit
TTCGTACCTCAGTGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT1590.1468266060891487No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1550.14313285499256634No Hit
TTCGCACATGAGCGTCAGTTGTGTGCCAGGAAGCCGCCTTCGCCACTGGT1520.14036254167012957No Hit
TTCGTCTTTCAGAGTCAGTTTCCGTCCAGCAAGCTGCCTTCGCTATTGGT1480.1366687905735472No Hit
TTCGTGCTTCAGCGTCAGTTATAGTATGGTATGCTGCCTTCGCAATCGGA1480.1366687905735472No Hit
TTCGCCCCTCAGCGTCAATACCGGTCCAGGTGGCCGCCTTCGCCACTGAT1470.13574535279940161No Hit
TTCGAGCCTCAGCGTCAGTTTCGCCCCGGCAGAATGCCTTCGCTGTCGGA1440.13297503947696485No Hit
TTCGTGCTTCAGTGTCAGTTATAGTTTAGTAAGCTGCCTTCGCAATCGGA1370.12651097505794573No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT1360.12558753728380015No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT1330.12281722396136337No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT1230.11358284621990748No Hit
TTCGTGTCTCAGCGTCAGTTGTCGTCCAGTAAATCGCCTTCGCCACTGGT1210.11173597067161628No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT1210.11173597067161628No Hit
TTCGAGCATGAGCGTCAGTTTCTGTCCAGTAAGCTGCCTTCGCCTTCGGT1190.10988909512332512No Hit
TTCGTGCTTCAGCGTCAGTTATAGCCCAGTATGCTGCCTTCGCAATCGGG1170.10804221957503393No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT1090.10065471738186924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)