FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40650
Sequences flagged as poor quality0
Sequence length200
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT672016.531365313653136No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA448211.025830258302582No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA406510.0No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT37409.200492004920049No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT19734.853628536285362No Hit
TTCGAGCATCAGTGTCAGTAACAGTCTAGTGAGCTGCCTTCGCAATCGGA11092.7281672816728166No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA10682.6273062730627306No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA9932.44280442804428No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA9552.3493234932349325No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT9292.2853628536285364No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA8162.007380073800738No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT6151.5129151291512914No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG5981.4710947109471093No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT5541.3628536285362853No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT5321.3087330873308733No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT5311.3062730627306274No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA4861.1955719557195572No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT4811.1832718327183271No Hit
TTCGAGCCTCAACGTCAGTGGCGGCTTAGCAGGCTGCCTTCGCAATCGGG4361.072570725707257No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT4050.996309963099631No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT3970.9766297662976631No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT3810.9372693726937269No Hit
TTCGAGCCTCAGCGTCAGTTACAGACCAGAGAGCCGCTTTCGCCACCGGT3360.8265682656826568No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT2990.7355473554735547No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT2940.7232472324723248No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT2840.6986469864698647No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG2400.5904059040590406No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT2180.5362853628536285No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT2040.5018450184501845No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA1990.48954489544895446No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT1940.47724477244772445No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT1690.41574415744157445No Hit
TTCGTGCTTCAGCGTCAGTTATAGCCCAGTATGCTGCCTTCGCAATCGGG1590.3911439114391144No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCTTTCGCCACTGGT1590.3911439114391144No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT1510.3714637146371464No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT1380.3394833948339483No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT1370.3370233702337023No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT1330.32718327183271834No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT950.23370233702337023No Hit
TTCGAGCCTCAACGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT720.1771217712177122No Hit
TTCGCGCCTCAGCGTCAGTTTCGGCCCAGAGAGCTGCCTTCGCCGTCGGT710.17466174661746617No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT690.16974169741697415No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGACCGCCTTCGCCACTGGT660.16236162361623616No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT650.15990159901599016No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT640.15744157441574416No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT630.15498154981549817No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGAAAGCCGCCTTCGCCACTGGT620.15252152521525214No Hit
TTCGGGACTTAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT530.1303813038130381No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATCGGT510.12546125461254612No Hit
TTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT480.11808118081180813No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAATCCGCCTTCGCCACTGGT470.1156211562115621No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA460.11316113161131611No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGA440.10824108241082411No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG430.1057810578105781No Hit
TTCGAGCCTCAACGTCAGTGGCGGCTTAGCAGGCTGCCTTCGCAATCGGA420.1033210332103321No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGA410.10086100861008611No Hit
TTCGTGCTTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT410.10086100861008611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)