FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33373
Sequences flagged as poor quality0
Sequence length200
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA548016.420459653012916No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCGTTAAGTCAGCT17385.207802714769424No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGACGGGTCCTTAAGTCAGTT16604.974080843795883No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT12503.745542804063165No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT8362.5050190273574446No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT6031.8068498486800706No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA6021.8038534144368203No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTGTCAAGTCAGCG5581.6720103077337969No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT5521.6540317022742934No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT5161.5461600695172746No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT5141.5401672010307734No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA4621.3843526203817458No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT4101.228538039732718No Hit
TCCGGAATGACTGGGCGTAAAGGGTGTGTAGGCGGGCTCGCAAGTTGGAT3891.165612920624457No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT3711.1116771042459472No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCCCAAGTCCCTC3701.1086806700026968No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT3641.0907020645431935No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT3611.081712761813442No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGGGATTAAGTCAGTT3511.0517484193809368No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGGCAAGTCAGAT3351.0038054714889282No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT3250.9738411290564228No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA2960.8869445360021575No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGCGATCAAGTTGGAA2800.8390015881101489No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG2770.8300122853803974No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT2750.8240194168938962No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT2680.8030443771911425No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT2560.7670871662721361No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTCAGATAAGTTAGAG2300.6891798759476223No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCGATTAAGTTAGAT2200.6592155335151171No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCCGTCCTTTAAGCGTGCT2170.6502262307853655No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA2140.6412369280556138No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA2100.6292511910826116No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTATTAAGTCAGTT2050.614269019866359No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA2000.5992868486501064No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGCATGATAAGTCTGAT1980.5932939801636054No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA1970.5902975459203548No Hit
TCCGGAATTATTGGGCGTAAAGCGTGCGTAGGCGGCCACTTAAGTCTGTC1780.5333652952985947No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAATACAAGTTAGTT1700.5093938213525905No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGAT1620.4854223474065862No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGGATACAAGTCAGAT1610.4824259131633356No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA1610.4824259131633356No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGGAGATAAGTTGGAA1580.47343661043358404No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT1540.4614508734605819No Hit
TCCGGAATTACTGGGCGTAAAGGGCGCGTAGGCGGCCGGATAAGTTAGAT1440.43148653102807655No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAGACAAGTTGGTT1430.428490096784826No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT1410.42249722829832503No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT1380.41350792556857335No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCTGATAAGTTGGAT1330.39852575435232074No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGAATGCAAGTCAGAT1250.3745542804063165No Hit
TCCGGATTTACTGGGTGTAAAGGGTGTGTAGGCGGGAAGGCAAGTCAGAT1230.3685614119198154No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGACAACAAGTTAGTT1220.3655649776765649No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA1160.3475863722170617No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATTGCAAGTCAGAT1090.32661133251430796No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGGAGACAAGTCAGAT1070.3206184640278069No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATATTTAAGTCAGTT1040.31162916129805535No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGCAGGCGGGATTGCAAGTTGGAT1030.30863272705480477No Hit
TCCGGATTTACTGGGTGTAAAGGGTGAGTAGGCGGGTCTGCAAGTCAGAT1030.30863272705480477No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGACTGTCAAGTCAGTC1020.30563629281155424No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG1010.3026398585683037No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGAT970.2906541215953016No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTTGGGA960.28765768735205105No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG950.2846612531088005No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG920.2756719503790489No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT890.26668264764929733No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA880.2636862134060468No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA850.25469691067629524No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT840.25170047643304466No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA840.25170047643304466No Hit
TCCGGATTTACTGGGTGTAAAGGGTGTGTAGGCGGGACTGCAAGTCAGAT800.23971473946004254No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGTATCAAGTCTGAT760.22772900248704042No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGCTTTTAAGTCAGTT750.22473256824378987No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT750.22473256824378987No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCT750.22473256824378987No Hit
TCCGGAATCATTGGGCGTAAAGCGCGCGTAGGCGGCTTGTTAAGCAGGAA670.20076109429778563No Hit
TCCGGAATGACTGGGCGTAAAGGGTGCGTAGGTGGTTTAGCAAGTTGGCA640.19177179156803403No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCGAGGCAAGTCAGGC620.185778923081533No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGATAGCAAGTCAGCT620.185778923081533No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT610.18278248883828244No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGCAGGCAAGTCAGGC600.1797860545950319No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA600.1797860545950319No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACAGCAAGCCAGAT590.1767896203517814No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGTGGCAAGGCAAGCCAGAA580.17379318610853084No Hit
TCCGGAATGACTGGGCGTAAAGGGTGCGTAGGTGGTATATCAAGTTGGCA570.17079675186528032No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT570.17079675186528032No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTGAGACAAGTCTGAA550.16480388337877927No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG520.15581458064902767No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTTGCAAGTCTGAT510.15281814640577712No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA490.14682527791927608No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT480.14382884367602553No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGATGGCAAGTCAGAT480.14382884367602553No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGGTTGTTAAGTCAGTT470.140832409432775No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG460.13783597518952445No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGAATGCAAGTCAGAT450.13483954094627393No Hit
TCCGGAATGACTGGGCGTAAAGGGCGTGTAGGCGGCAGTATAAGTCCGGA440.1318431067030234No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCTTTGCAAGTCAGAT440.1318431067030234No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGATAAGACAGAT440.1318431067030234No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT400.11985736973002127No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGTAAGTTGGAA400.11985736973002127No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGAGAGACAAGTCAGAT400.11985736973002127No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAA400.11985736973002127No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGGAGACAAGTTGGAA380.11386450124352021No Hit
TCCGGAATTATTGGGCGTAAAGAGTGCGTAGGTGGTTACCTAAGCAGGGG380.11386450124352021No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGGTAAGTTTGAA370.11086806700026969No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGAT370.11086806700026969No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGCAGGTGGCCATGCAAGTCAGAA370.11086806700026969No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGAGACAAGTTGAAT350.10487519851376861No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT350.10487519851376861No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCCGCAGGTTAAGCGTGTT350.10487519851376861No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGTAGGCTGTATGTCAAGTCAAGG340.10187876427051809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)