FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37042
Sequences flagged as poor quality0
Sequence length200
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT415411.214297284163923No Hit
TTCGTGCCTCAGCGTCAGTCGGGCGCCGGTACGCTGCCTTCGCAATCGGA32278.711732627827871No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT21295.7475298310026455No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACTGGT18585.015927865666001No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT16634.489498407213433No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT10982.9642027968252256No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT10332.78872631067437No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT9002.429674423627234No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT7832.1138167485556933No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT7482.019329409859079No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT5381.4524053776793908No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT5281.425408995194644No Hit
TTCGTGCCTCAACGTCAGATATAGCTTGGTAAGCTGCCTTCGCAATCGGT5021.3552184007343016No Hit
TTCGAGCCTCAGCGTCAGTTAGAGCCCAGCAAGCCGCCTTCGCCACTGGT4861.3120241887587065No Hit
TTCGAGCCTCAACGTCAGTCGCGGCTTAGCAGGCTGCCTTCGCAATCGGG4681.2634307002861616No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT4481.2094379353166675No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT4221.1392473408563253No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT4171.1257491496139518No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA4171.1257491496139518No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT3961.0690567463959828No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA3911.0555585551536093No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT3791.023162896171913No Hit
TTCGTGCTTCAGTGTCAGAATCCAGACCAGACGGCCGCCTTCGCCACCGG3731.0069650666810648No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT3520.950272663463096No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT3320.8962798984936019No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGAAAGCCGCCTTCGCCACTGGT3120.8422871335241078No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT3080.831488580530209No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT2800.7558987095729173No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT2700.7289023270881702No Hit
TTCGCACCTCAGCGTCAATACCGGTCCAGGTGGCCGCCTTCGCCACTGAT2580.6965066681064738No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT2570.693807029857999No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCGCTGGT2560.6911073916095243No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT2530.6830084768641002No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTGCGGCAGGCTGCCTTCGCAATCGGA2430.6560120943793533No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT2100.5669240321796879No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCTACTGGT2060.556125479185789No Hit
TTCGAGCCTCAACGTCAGTTACAATCCAGTAAGCCGCCTTCGCCACTGGT2060.556125479185789No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA1980.5345283731979914No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGAAGGCTGCCTTCGCCATCGGT1890.5102316289617191No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1880.5075319907132444No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT1770.47783596998002265No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT1610.4346417580044274No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA1600.4319421197559527No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG1480.39954646077425626No Hit
TTCGGGAATGAGCGTCAGTTGCGGCCCAGACCGTCGCCTTCGCCACTGGT1440.38874790778035745No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1200.3239565898169645No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGACCGCCTTCGCCACTGGT1190.3212569515684898No Hit
TTCGTGCTTCAGCGTCAGTTGTAGTATGGTATGCTGCCTTCGCAATCGGA1190.3212569515684898No Hit
TTCGAGCCTCAGCGTCAGTTACAGCCCAGTAGGCCGCCTTCGCCACTGGT1160.3131580368230657No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT1110.2996598455806922No Hit
TTCGAGCCTCAGCGTCAGTAATGCCCCGGTACAATGCCTGCGCGATCGGA1100.29696020733221745No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT1080.2915609308352681No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTTGGCCGCCTTCGCCACCGGT1080.2915609308352681No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT1080.2915609308352681No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT1010.27266346309594514No Hit
TTCGCGCCTCAACGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT980.264564548350521No Hit
TTCGCTCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT980.264564548350521No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGCAAATCGCCTTCGCCACTGGT910.24566708061119813No Hit
TTCGTGCCTCAGCGTCAGTTACGGCCCAGTATGTCGCTTTCGCCACCGGT810.21867069812645107No Hit
TTCGTGCCTCAGCGTCAGTTAGAGCCCAGTAGGCCGCCTTCGCCACTGGT790.21327142162950166No Hit
TTCGTGCCTCAACGTCAGCTATAGCTTGGTAAGCTGCCTTCGCAATCGGT750.20247286863560285No Hit
TTCGTGCATGAGCGTCAGTTTTGCCCCAGGGGGCTGCCTTCGCCATCGGT720.19437395389017872No Hit
TTCGAGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACCGAT710.191674315641704No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATTGGA710.191674315641704No Hit
TTCGTGACTGAGCGTCGGTATCGGTCTGGCGGACCGCCTTCGCCACCGGT710.191674315641704No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT700.18897467739322932No Hit
TTCGAGCCTCAACGTCAGTTACGGTCCAGCAAGCTGCCTTCGCAATCGGA660.1781761243993305No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT650.1754764861508558No Hit
TTCGAGCTTCAGCGTCAGTTGCGCTCCAGTGAGCTGCCTTCGCAATCGGA650.1754764861508558No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC620.16737757140543166No Hit
TTCGTGCTTCAGTGTCAGTTATGGCTTGGCAAGCTGCCTTCGCAATCGGA610.16467793315695697No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAGGCCGCCTTCGCCACTGGT610.16467793315695697No Hit
TTCGCCTCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT560.15117974191458344No Hit
TTCGTCCCTGAGTGTCAGTCATCGTCCAGAAATCTGCCTTCGCTTTCGAT560.15117974191458344No Hit
TTCGCATCTCAGCGTCAGTTACGAGCCAGATAGCCGCCTTCGCCACTGAT560.15117974191458344No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA560.15117974191458344No Hit
TTCGAGCCTCAACGTCAGTCACTGTCCAGTAAGCCGCCTTCGCCACTGGT520.14038118892068463No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGGT520.14038118892068463No Hit
TTCGAGCATCAGCGTCAGTGGTGCTGCGGCATGCTGCCTTCGCAATCGGA520.14038118892068463No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT510.1376815506722099No Hit
TTCGCCCCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT510.1376815506722099No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT500.13498191242373522No Hit
TTCGCATCTCAGCGTCAGCCACAGTCCAGAAAGGCGCCTTCGCCACTGGT480.1295826359267858No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT470.1268829976783111No Hit
TTCGAGCCTCAGCGTCAGTTACAGTCCAGAGAATCGCCTTCGCCACTGGT470.1268829976783111No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT470.1268829976783111No Hit
TTCGTGCATGAGCGTCAGTATTATCCCAGGAGGCTGCCTTCGCCATCGGT440.118784082932887No Hit
TTCGCTCCTCAGCGTCAGTCGAGGCCCAGAAGGCCGCCTTCGCCACCGGT440.118784082932887No Hit
TTCGTGCTTCAGTGTCAGCATCCAGACCAGACGGCCGCCTTCGCCACCGG430.1160844446844123No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGCAGGCTGCCTTCGCCATCGGT420.1133848064359376No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT410.11068516818746289No Hit
TTCGTGCCTCAGCGTCAGTTCCGGTCCAGCAGGCCGCCTTCGCCACTGGT410.11068516818746289No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTCCGGTAAGCTGCCTTCGCAATCGGA410.11068516818746289No Hit
TTCGAGCCTCAGCGTCAGTTGCGCTCCGGACAGCTGCCTTCGCAATCGGA400.10798552993898818No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGACCGCCTTCGCCACTGGT400.10798552993898818No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGCT390.10528589169051347No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT390.10528589169051347No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT390.10528589169051347No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT380.10258625344203877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)