FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37042
Sequences flagged as poor quality0
Sequence length200
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA380610.274823173694724No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGACAGTTAAGTCAGCG31828.59024890664651No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCCGAGCAAGTCAGTT20835.623346471572809No Hit
TCCGGAATTACTGGGTGTAAAGGGAGTGTAGGCGGGATATCAAGTCAGAA16994.586685384158522No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACAGCAAGTCTGAT13283.5851195939744076No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGCGATCAAGTTGGAA12533.3826467253388044No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT10642.8724150963770856No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATAGCAAGTCAGAT8492.291992872955024No Hit
TCCGGAATAATTGGGCGTAAAGGGCGCGTAGGCGGCTCGGTAAGTCTGGA7391.9950326656228066No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTCAGATAAGTTAGAG5911.5954862048485503No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT5611.514497057394309No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA5351.4443064629339668No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCTTAATAAGTCGAGC5141.387614059715998No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG4841.3066249122617568No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGACGGGTCGTTAAGTCAGCT4831.3039252740132823No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGATAGCAAGTCAGCT4681.2634307002861616No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT4321.1662437233410723No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGTGGCAGATCAAGTCTGGA4061.09605312888073No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT3991.077155661141407No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT3961.0690567463959828No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGCTTTTAAGTCAGTT3500.9448733869661465No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT3350.9043788132390259No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCTATGCAAGTCAGAA3250.8773824307542789No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGAT3170.8557853247664813No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT3020.8152907510393607No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGAT2950.7963932833000379No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCTGTTATGTAAGTCAGGG2750.7424005183305437No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCACAGCAAGTCAGAA2640.712704497597322No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGACTGTCAAGTCAGTC2580.6965066681064738No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG2330.6290157118946061No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGTTATGTAAGATAGCG2280.6155175206522325No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCGGTTGGGAAAGCGTGCC2180.5885211381674855No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA2170.5858214999190109No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA2060.556125479185789No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG2000.5399276496949409No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTTTCTTAAGTCAGCG1970.5318287349495168No Hit
TCCGGATTTACTGGGCGTAAAGGGTGCGTAGGCGGTCTTTTAAGTCAGGA1870.5048323524647698No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGGA1800.4859348847254468No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA1760.475136331731548No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTTGGTAAGTTGGAT1750.47243669348307327No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGTCAAGCGGGGT1710.46163814048917445No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGACTGCAAGTCAGAT1600.4319421197559527No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA1590.42924248150747796No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTTGGGA1560.4211435667620539No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG1540.4157442902651045No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAA1460.3941471842773069No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACTATTAAGTCAGCT1450.3914475460288321No Hit
TCCGGAATCATTGGGCGTAAAGAGGGAGCAGGCGGCCGCAAGGGTCTGTG1420.383348631283408No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT1320.356352248798661No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT1310.35365261055018626No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATGCCAAGTCAGCT1270.34285405755628745No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGAGACAAGTTGAAT1220.32935586631391395No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAA1220.32935586631391395No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA1210.3266562280654392No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTCCTGCAAGTCTGAT1200.3239565898169645No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGGTTGTTAAGTCAGCG1150.310458398574591No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT1150.310458398574591No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGCAGTCAAGTCAGCG1130.3050591220776416No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGATTGCAAGTCAGAT1080.2915609308352681No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGCAGGCAAGTCAGGC1010.27266346309594514No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT830.22406997462340048No Hit
TCCGGATTTACTGGGTGTAAAGGGCGAGTAGGCGGGCTGGTAAGTTGGAA820.22137033637492579No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGAT750.20247286863560285No Hit
TCCGGAATCATTGGGCGTAAAGAGTGAGCAGGCGGTCCGGGAAGTCCGCG730.19707359213865344No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGCTAAGTCTGAA710.191674315641704No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGAA710.191674315641704No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG700.18897467739322932No Hit
TCCGGAATTATTGGGCGTAAAGGGCGCGTAGACGGCTGAGTAAGTTACTG700.18897467739322932No Hit
TCCGGAATGACTGGGCGTAAAGGGTGCGTAGGCGGCATGTCAAGTTGGCA670.1808757626478052No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTAATTTAAGTCAGCG670.1808757626478052No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCTTTAAGTCAGTT660.1781761243993305No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCTGATAAGTTGGAT650.1754764861508558No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA650.1754764861508558No Hit
ATCGGAATTACTGGGCGTAAAGCGCGTGTAGGCTGTATGGCAAGTTGGAG640.17277684790238107No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTTGCAAGTCTGAC630.17007720965390638No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGAAGAGCAAGTCAGTA620.16737757140543166No Hit
TTCGGATTCATTGGGCGTAAAGGGTCCGCAGGTGGTTTGATAAGTCTGAC610.16467793315695697No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGACGGATGGGCAAGTCTGAT600.16197829490848226No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAGTGCAAGTCTGAA580.15657901841153285No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGGAGATTAAGCGTGTT520.14038118892068463No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTTTGCAAGTCTGAT480.1295826359267858No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT470.1268829976783111No Hit
TCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGAT450.1214837211813617No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGTCTCAAAAGCGTGTT440.118784082932887No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCACAGCAAGTCAGAT430.1160844446844123No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT430.1160844446844123No Hit
TTCGGAATTACTGGGTGTAAAGGGCATGTAGGCTGAGATAAAAGTTAGCG420.1133848064359376No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGGT420.1133848064359376No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCCGTCCTTTAAGCGTGCT420.1133848064359376No Hit
TCCGGAATTATTGGGCGTAAAGAGTGCGTAGGTGGTTATCTAAGCGTAAG410.11068516818746289No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAGAACAAGTTAGTT410.11068516818746289No Hit
CTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT400.10798552993898818No Hit
TCCGGATTCATTGGGCGTAAAGCGCTCGTAGGCTGCCCGCTAGGTCCGGG400.10798552993898818No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGAGAGACAAGTCAGAT400.10798552993898818No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGACGGGTCGTTAAGTCAGCT380.10258625344203877No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCAGAGCAAGTCAGGC380.10258625344203877No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGGATGCAAGTTGAAT380.10258625344203877No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGTCTGAA380.10258625344203877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)