FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61606
Sequences flagged as poor quality0
Sequence length200
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA59499.656526961659578No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT55248.966659091646918No Hit
TTCGTGCATCAGCGTCAGTTAAGGCCCAGCAGGCCGCCTTCGCCACTGGT41696.767198000194787No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA41306.70389247800539No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAAGCTGCCTTCGCAATCGGA31885.174820634353797No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT25074.069408823815862No Hit
TTCGAGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT15322.4867707690809335No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT15012.4364509950329514No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAAGCCGCCTTCGCCACTGGT12802.0777197026263674No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT12762.0712268285556603No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT12612.0468785507905074No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT12612.0468785507905074No Hit
TTCGTGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACCGGT12211.9819498100834336No Hit
TTCGTGCCTCAACGTCAGTTACTGTCCAGAAAGTCGCCTTCGCCACTGGT11601.8829334805051456No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATTGGT11241.824497613868779No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT10351.6800311657955393No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA10221.6589293250657402No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA8981.457650228873811No Hit
TTCGTGCCTCAGCGTCTGTCGCACCCTAGCAGGCTGCCTTCGCAATCGGG8601.3959679252020907No Hit
TTCGTGCCTCAGCGTCAGTAAAAGCCCAGCAAGCCGCCTTCGCCACCGGT7321.1881959549394538No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT7021.1394993994091485No Hit
TTCGTCCCTCACCGTCAGAATCGTTCCAGTCAGACGCCTTCGCAACAGGC6070.9852936402298477No Hit
TTCGTGCCTCAGCGTCAGTGTCAGTCCAGAAAGCCGCCTTCGCCACTGGT5690.9236113365581274No Hit
TTCGCTCCTCAGCGTCAGTGACGGCCCAGAGACCTGCCTTCGCCATCGGT5450.884654092133883No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT5390.874914781027822No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA4940.8018699477323638No Hit
TTCGTGCATGAGCGTCAGTTTTGCCCCAGGGGGCTGCCTTCGCCATCGGT4720.7661591403434731No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT4160.6752589033535695No Hit
TTCGCGCCTCAGCGTCAGTTATGGCCCAGAAAGCTGCCTTCGCCATCGGT3560.5778657922929584No Hit
TTCGTGCCTCAGCGTCAGTTAATGTCCAGGAACCCGCCTTCGCCACGAGT3140.5096906145505309No Hit
TTCGTGTCTCAGCGTCAGTTGTCGTCCAGTAAGCCGCCTTCGCCACCGGT3060.496704866409116No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT2800.4545011849495179No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT2660.43177612570204205No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT2550.41392072200759666No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT2500.40580462941921236No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT2440.39606531831315134No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT2290.3717170405479986No Hit
TTCGTGCCTCAGCGTCCGATATAGATTTGTAAGCTGCCTTCGCAATCGGT2290.3717170405479986No Hit
TTCGTGCCTCAACGTCAGTTACGGTCCAGAAAGTCGCCTTCGCCACTGGT2180.35386163685355326No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT2150.34899198130052267No Hit
TTCGTGCTTCAGTGTCAGTTGCAGACCAGGCGGCCGCCTTCGCCACTGGT2050.33275979612375417No Hit
TTCGAGCATCAGTGTCAGTAACAGTCTAGTGAGCTGCCTTCGCAATCGGA2040.33113657760607734No Hit
TTCGGGACTGAGCGTCAGCTGCACCCCAGACCGCCGCCTTCGCCACTGGT2030.3295133590884005No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT1980.32139726650001627No Hit
TTCGCGCCTCAGCGTCAGTAACAGTCCAGTAAGCCGCCTTCGCCACTGGT1910.31003473687627825No Hit
TTCGTGTCTCAGCGTCAGTAATAGCCCAGCAAGCCGCCTTCGCCACTGGT1870.3035418628055709No Hit
TTCGCGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1840.29867220725254034No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1800.292179333181833No Hit
TTCGTGCCTCAGCGTCAGTTACTGTCCAGATAGTCGCCTTCGCCACTGAT1620.26296139986364964No Hit
TTCGCGCCTCAGCGTCAGTTACTGTCCAGAAAGCCGCCTTCGCCACTGGT1600.2597149628282959No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1490.2418595591338506No Hit
TTCGAGCCTCAGCGTCAGTTAATGTCCAGAAAGCCGCCTTCGCCTCTGGT1410.2288738109924358No Hit
TTCGGGACTGAGCGTCAGTTGCAGCCCAGATCGTCGCCTTCGCCACTGGT1400.22725059247475896No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTAGACCGCCTTCGCCACTGGT1390.2256273739570821No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA1390.2256273739570821No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1290.20939518878031363No Hit
TTCGTGCCTCAGCGTCAGTTTCAGTCCAGTTAACCGCCTTCGCCACTGGT1260.20452553322728306No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT1210.1964094406388988No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT1200.19478622212122196No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT1080.17530759990909978No Hit
TTCGCGCCTCAGCGTCAGTTGCTGTCCAGCAGGCCGCCTTCGCCACTGGT1040.16881472583839238No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT1030.16719150732071553No Hit
TTCGTGTCTCAGCGTCAGTAATCGTCCAGTAAACCGCCTTCGCCACTGGT970.15745219621465442No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT950.1542057591793007No Hit
TTCGAGCCTCAACGTCAGTCACTGTCCAGTAAGCCGCCTTCGCCACTGGT890.1444664480732396No Hit
TTCGTGCCTCAGCGTCAGTTGCAGTCCAGAAAGCCGCCTTCGCCACTGGT890.1444664480732396No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA890.1444664480732396No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGCAAGTCGCCTTCGCCACTGGT870.14122001103788592No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTTGGCCGCCTTCGCCACTGGT850.13797357400253224No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT820.13310391844950167No Hit
TTCGTGCCTCAACGTCAGTTGCGGTCCAGAAAGTCGCCTTCGCCACTGGT800.12985748141414796No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT660.10713242216667208No Hit
TTCGCGCCTCAGCGTCAGTTACGGCCCAGTAAGTCGCCTTCGCCACTGGT650.10550920364899521No Hit
TTCGTCCCTCACCGTCAGCATCGTTCCAGTCAGACGCCTTCGCAACAGGC650.10550920364899521No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT650.10550920364899521No Hit
TTCGTGCTTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT630.10226276661364153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)