FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61606
Sequences flagged as poor quality0
Sequence length200
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT58079.426029932149465No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAATTAAGTTAGTT50798.24432685128072No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCAATGCAAGTCAGAT44197.173002629613999No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTATTAAGTCAGTT42886.96036100379833No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT42366.8759536408791355No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGACTGCAAGTTGGAT25454.131091127487582No Hit
TCCGGAATTACTGGGCGTAAAGGGAGCGTAGGCGGTCGATTAAGTTAGAT14732.3910008765379995No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG13012.1118072914975814No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT12992.108560854462228No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGCAATTTAAGTTGGAT12432.017660617472324No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGAGAGACAAGTCAGAT12021.9511086582475732No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT10321.675161510242509No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCCGAGCAAGTCAGTT10231.660552543583417No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGCCAATCAAGTCAGAT8661.4057072363081518No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGAAGCAAGTCAGAA8611.3975911437197677No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG8181.327792747459663No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT7331.1898191734571306No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGGCGGGATGTAAAGTCAGAT7301.1849495179041003No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAGGTAAGTTAGTT6801.103788592020258No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT6621.0745706587020745No Hit
GCAGTTTTTATTGGGCCTAAAGCGTCCGTAGCCGGTTTAATAAGTCTCTG6361.0323669772424764No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGCGGTTGCTTAAGTTGGAT5810.9430899587702497No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGCTCGTCGCGTCCGGT4830.7840145440379185No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT4390.712592929260137No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT3690.5989676330227576No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAAAATAAGTTAGTT3520.571372918222251No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA3400.551894296010129No Hit
TCCGGAATCATTGGGCGTAAAGCGCGCGTAGGCGGCTTGTTAAGCAGGAA3380.5486478589747752No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAGAACAAGTTAGTT3370.5470246404570983No Hit
TCCGGAATTATTGGGCGTAAAGAGCATGTAGGCGGCCTATTAAGTCGGGC3290.5340388923156836No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA3270.5307924552803298No Hit
TTCGGAATCACTGGGCGTAAAGCGTGCGTAGGCTGTTTCGTAAGTCGTGT3150.5113138330682077No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGAGATGCAAGTCAGAT3120.5064441775151771No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGGTAAGACAGAT2980.4837191182677012No Hit
TCCGGATTTACTGGGTGTAAAGGGTGTGTAGGCGGGATTGCAAGTCAGAT2960.48047268123234743No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA2740.44476187384345683No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA2710.43989221829042624No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT2600.42203681459598086No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCAGATTAAGTTAGAG2580.41879037756062726No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGGATGGCAAGTCAGAT2370.3847027886894133No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAGATAAGTTAGTT2360.38307957017173655No Hit
TCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCACGATAAGTCTGTA2080.33762945167678476No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAGTTTAAGTTAGTT2080.33762945167678476No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTAGTAAGTCAGCG2050.33275979612375417No Hit
TCCGGAATCATTGGGCGTAAAGAGGGAGCAGGCGGCAGTGCAGGTCTGCG2040.33113657760607734No Hit
TCCGGATTTACTGGGTGTAAAGGGCGAGTAGACGGAACTGCAAGTCAGAT1770.2873096776288024No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT1730.28081680355809496No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGGCAAGTTGGAA1710.2775703665227413No Hit
TCCGGAATGACTGGGCGTAAAGGGTGTGTAGGCGGTTCGGTAAGTTAGAA1630.26458461838132646No Hit
TCCGGAATCATTGGGCGTAAAGAGGGAGCAGGCGGCAATAGAGGTCTGCG1560.25322208875758856No Hit
TCCGGATTTATTGGGCGTAAAGCGTGTGTAGGCGGTTTATTAAGTTTAAG1500.24348277765152745No Hit
TCCGGAATGACTGGGCGTAAAGGGAGCGTAGGCGGCACAACAAGTTAGGA1400.22725059247475896No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGATGGCAAGTCAGAT1320.21426484433334417No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCATGGCAAGTCAGAA1310.2126416258156673No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGTAGGCGGGGATACAAGTCAGAT1300.21101840729799043No Hit
TCCGGAATTACTGGGCGTAAAGGGTGAGTAGGCGGTTTAAAAAGTCTGCA1240.20127909619192938No Hit
TCCGGAATTATTGGGCGTAAAGAGTGCGTAGGTGGTAACTTAAGCGCGGG1240.20127909619192938No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAGGGCAAGTCAGAT1220.19803265915657567No Hit
TCCGGATTTATTGGGTGTAAAGGGTGCGTAGACGGGAGTGCAAGTTGGTT1150.1866701295328377No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGGTAAGTTTGAA1120.18180047397980714No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCGGCTTGGTAAGTCAGGG1110.18017725546213031No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGTTTGCAAGTCAGAT1010.16394507028536182No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA970.15745219621465442No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT970.15745219621465442No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAAGGCAAGTCAGAT930.150959322143947No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT920.14933610362627017No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT910.14771288510859332No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGGATAAGACAGAT900.1460896665909165No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGAT880.14284322955556275No Hit
TCCGGAATGACTGGGCGTAAAGGGCGCGTAGGCGGCTGTGTAAGTCTGGA850.13797357400253224No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT850.13797357400253224No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTATTAAGTTAGTG840.13635035548485536No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA820.13310391844950167No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT810.13148069993182482No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAAGTGCAAGTCTGAA810.13148069993182482No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAGACAAGTTAGTT790.1282342628964711No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAAGGCAAGTCAGAT790.1282342628964711No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA760.12336460734344058No Hit
TCCGGAATGATTGGGCGTAAAGGGCGCGTAGGCGGCCTGATAAGTTTGAA740.12011817030808686No Hit
TCCGGAATTATTGGGTGTAAAGGGTGCGTAGACGGGAAATTAAGTTAGTT710.11524851475505633No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT690.11200207771970264No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGCGGCACCGTAAGTCTGTT640.10388598513131839No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCTATGCAAGTCAGAA640.10388598513131839No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT630.10226276661364153No Hit
TCCGGATTTACTGGGTGTAAAGGGTGTGTAGGCGGGTCTGCAAGTCAGAT620.10063954809596469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)