FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43487
Sequences flagged as poor quality0
Sequence length200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT1023223.528870696989905No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT491011.290730563156806No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA34247.873617402902017No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT24195.562581921033872No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA15813.635569250580634No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT14303.2883390438521856No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA13053.0008968197392325No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT8401.9316117460390463No Hit
TTCGTGCCTCAGTGTCAGTAGCAGTCCAGTAAGTCGCCTTCGCCACTGGT8301.90861636811001No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT8071.8557269988732263No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT7751.7821417895003107No Hit
TTCGAGCCTCAATGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACCGGT6351.4602064984938026No Hit
TTCGCGCCTCAGCGTCAGTTTTCGTCCAGAAAGTCGCCTTCGCCACCGGT6341.4579069607008992No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT6231.4326120449789592No Hit
TTCGTGCCTCAGCGTCAGTATATGGCCAGATAGCCGCCTTCGCCACCGGT5521.2693448616828018No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT4661.07158461149309No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT4561.0485892335640536No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT4220.9704049486053303No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT3910.8991192770253179No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT3780.8692252857175709No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT3550.8163359164807874No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT2940.6760641111136662No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCTAGTGAGCTGCCTTCGCAATCGGA2770.6369719686343045No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA2590.5955802883620392No Hit
TTCGAGCCTCAGCGTCAGTTATGCCCCGGCACAATGCCTTCGCGATCGGA2420.5564881458826776No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT2420.5564881458826776No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG2410.5541886080897739No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT2400.5518890702968704No Hit
TTCGTGCCTCAGCGTCAGTTGACAGCCAGATACTCGCCTTCGCCACTGGT2380.5472899947110631No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT2010.46220709637362895No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1930.4438107940303999No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1890.4346126428587854No Hit
TTCGTGCCTCAACGTCAGTTACAGTCCAGTAAGTCGCCTTCGCCACTGGT1730.3978200381723274No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT1720.3955205003794237No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT1480.3403315933497367No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA1460.33573251776392943No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA1450.33343297997102583No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT1420.32653436659231494No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT1420.32653436659231494No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGCAAGCCGCCTTCGCCACTGGT1420.32653436659231494No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1350.31043760204198956No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGCCCGCCTTCGCCACTGGT1170.26904592176972425No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT1120.25754823280520617No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGCAGGCCGCCTTCGCCACTGAT1060.2437510060477844No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA1060.2437510060477844No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGTAGGCCGCCTTCGCCACTGGT990.22765424149745903No Hit
TTCGCACCTCAGCGTCAATACCGGTCCAGGTGGCCGCCTTCGCCACTGAT880.2023593257755191No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT850.19546071239680823No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACCGGT820.18856209901809737No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG810.18626256122519375No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT760.1747648722606756No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT680.1563685699174466No Hit
TTCGCGCCTCACCGTCAGTTGTCGTCCAGCAATCCGCCTTCGCCACTGGT640.1471704187458321No Hit
CTCGTGCATCAGCGTCAGTTACAGTCTGGTAAGCTGCCTTCGCTATCGGA640.1471704187458321No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT620.14257134316002482No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT600.1379722675742176No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAAAGCCGCCTTCGCCACTGGT560.12877411640260308No Hit
TTCGAGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT560.12877411640260308No Hit
TTCGAGCCTCAGCGTCAGTAAAAGCCCAGTAAGCCGCCTTCGCCACCGAT550.12647457860969943No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT550.12647457860969943No Hit
TTCGTCTTTCAGAGTCAGTTTCTGTCCAGCAAGCTGCCTTCGCTATTGGT540.12417504081679583No Hit
TTCGAGCCTCAGCGTCAATATACAGCCAGATAGTCGCCTTCGCCACTGGT540.12417504081679583No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT540.12417504081679583No Hit
TTCGAGCCTCAATGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT520.11957596523098858No Hit
TTCGCGCCTCACCGTCAGTTACCGTCCAGTAATCCGCCTTCGCCACTGGT520.11957596523098858No Hit
TTCGGGACTTAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT520.11957596523098858No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGTG510.11727642743808495No Hit
TTCGTGCCTCAACGTCAGCTATAGTTTGGTAAGCTGCCTTCGCAATCGGT500.11497688964518132No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCGCTGGT460.10577873847356682No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT460.10577873847356682No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT450.1034792006806632No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)