FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40098
Sequences flagged as poor quality0
Sequence length200
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT1180629.442864980797047No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT37289.297221806573894No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTCTTAAGTCTGAT36379.07027781934261No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGCTCGTCGCGTCCGGT34838.68621876402813No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATAGGTCAGTCTGTC21795.434186243702928No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATCAGTCAGTCTGTC20905.212230036410793No Hit
TTCGGAATTACTGGGCGTAAAGGGTGTGTAGGCGGTTATGTAAGATAGCG17104.264551847972467No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG15103.7657738540575587No Hit
TCCGGAATGACTGGGCGTAAAGGGCGCGTAGGCGGTCTATTAAGTCTGAT11552.8804429148585964No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT7581.8903685969375033No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGCTCGTCGCGTCCGGT7331.8280213476981395No Hit
TCCGGATTTACTGGGCGTAAAGGGTGCGTAGGCGGTCTTTCAAGTCAGGA7231.8030824480023941No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT5831.4539378522619582No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTTTGTAAGATAGAT5471.3641578133572747No Hit
TCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGATGTTTAAGTGGGAT4621.1521771659434386No Hit
TCCGGATTTATTGGGCGTAAAGAGAGTGCAGGCGGTTTTCTAAGTCTGAT3410.850416479624919No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT2840.70826475135917No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATCGGTCAGTCTGTC1950.4863085440670357No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGAT1860.4638635343408649No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGG1400.34914459574043594No Hit
TCCGGAATTATTGGGCGTAAAGCGAGCGCAGGCGGTTTCTTAAGTCTGAT950.23691954710958152No Hit
TCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGATTTTTAAGTGAGAT840.20948675744426157No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGAT740.18454785774851612No Hit
CCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGTG670.16709062796149435No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGGCTTCTAAGCGGAAC480.11970671853957804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)