FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53208
Sequences flagged as poor quality0
Sequence length200
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT1516228.495714930085704No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1243523.370545782589083No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA864816.253195008269433No Hit
TTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT44608.38219816568937No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT16073.020222522928883No Hit
TTCGGGACTGAGCGTCAGTTGCAGGCCAGATCGTCGCCTTCGCCACTGGT14192.666892196662156No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA13212.4827093670124794No Hit
TTCGAGCCTCAGCGTCAGTTAGTGCCCAGTAGGCCGCCTTCGCCACTGGT6431.2084648924973689No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTTGCAGGCTGCCTTCGCAATCGGG6311.185911892948429No Hit
TTCGCCCCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT4930.9265523981356186No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCCATCGGG3620.6803488197263569No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGT3060.5751014884979702No Hit
TTCGCACCTTAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT2530.4754924071568185No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGGAGGCTGCCTTCGCAATCGGG1960.3683656592993535No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGA1760.3307773267177868No Hit
TTCGCGCCTCAGCGTCGGTCTCGGCCCAGAGGGCCGCCTTCGCCACCGGT1720.32325966020147345No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGG1510.28379191099082846No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA1240.23304766200571342No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCCGCCTTCGCAATCGGG1010.18982107953691174No Hit
TCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGGG950.17854457976244173No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATTGGG920.17290632987520674No Hit
TTCGGGACTTAGCGTCAGTTGCAGGCCAGATCGTCGCCTTCGCCACTGGT890.16726807998797172No Hit
TTCGCCCCTTAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCGCCACCGGT840.15787099684258007No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCCGG780.14659449706811006No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTTGCAGGCTGCCTTCGCAATCGGT770.14471508043903172No Hit
TCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGTG770.14471508043903172No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTTGCAATCGGG760.14283566380995338No Hit
TTCGCACCTCAGCGTCAGTTACCGTCCAGGTGGCCGCCTTCGCCACCGGT700.1315591640354834No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTAGCAGGCTGCCTTCGCAATCGGG670.12592091414824838No Hit
TTCGGGCCTGAGCGTCAGTTGCAGGCCAGATCGTCGCCTTCGCCACTGGT570.10712674785746505No Hit
TCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGAG570.10712674785746505No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCCGGCTGCCTTCGCAATCGGG550.10336791459930837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)