FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38123
Sequences flagged as poor quality0
Sequence length200
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA639116.76415811977022No Hit
TCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGTTCAATAAGTCTGAT400310.500222962516066No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTT35679.356556409516564No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA17974.713689898486478No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTGATAAGTCTGAA15634.099887207197755No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTAGATAAGTCTGAA10862.848674028801511No Hit
TCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCAGGAAAGTCTGGA7962.087978385751384No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGATTGTTAAGTCAGTT7922.0774860320541406No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCACGGCAAGCCAGAT6551.7181229179235633No Hit
TCCGGAATTATTGGGCGTAAAGGGCTCGTAGGCGGTTCGTCGCGTCCGGT6101.600083938829578No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAGTGGCAAGTCTGAT5281.3849906880360938No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACAGTTAAGTCAGTT5091.335152007974189No Hit
ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT5091.335152007974189No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGACCGGCAAGTTGGAA5081.3325289195498782No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCATGCAAGTCAGAA4351.141043464575191No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGATTGTTAAGTCAGTT4091.0728431655431105No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGACAAGTCTGAT3951.0361199276027595No Hit
TCCGGATTTATTGGGCGTAAAGAGAGTGCAGGCGGTTTTCTAAGTCTGAT3680.9652965401463683No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGTTTGATAAGTTAGAG3530.9259502137817066No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTTGTGTAAGTCTGAT3520.9233271253573958No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGACGCTTAAGTCAGTT3220.8446344726280722No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGTAAGTCTGAA3210.8420113842037615No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGCGATCAAGTTGGAA2950.7738110851716811No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGTGGTGATTTAAGTCAGCG2360.6190488681373449No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTGCGGCAAGTCTGAT2310.6059334260157909No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACTGGTAAGTCAGTT2260.5928179838942371No Hit
TCCGGATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGAT2070.5429793038323322No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGTAAGTTGGAA2000.5246176848621567No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGCAGCCGGGTCTGCAAGTCAGAT1980.5193715080135352No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGAAGGCTAAGTCTGAT1960.5141253311649135No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTGTGGCAAGTCTGAT1930.5062560658919812No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGTTGTTAAGTCAGTT1910.5010098890433596No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGTGGCAGTGCAAGTCAGAT1850.4852713584974949No Hit
TCCGGAATTACTGGGCGTAAAGGGTGCGTAGGTGGTTTCTTAAGTCAGAG1750.4590404742543871No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGCAGGCTGCGAGGCAAGTCAGCG1720.45117120898145474No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCATCACAAGTCAGAA1700.4459250321328332No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCACGCCAAGTCAGCG1630.4275634131626577No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATGGCAAGCCAGAT1520.3987094404952391No Hit
TCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGATTGGTCAGTCTGTC1490.39084017522230674No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGGCTGGTAAGTCAGTT1480.38821708679799594No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGCCGGGAAGGCAAGTCAGAT1460.38297090994937444No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGGAGATTAAGCGTGTT1430.375101644676442No Hit
TCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTTTTAAGTCAGCG1320.34624767200902345No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCTGTGCAAGTCTGAA1260.33050914146315874No Hit
TCCGGAATTATTGGGCGTAAAGGGTGCGTAGGCGGCCTGTTAAGTTTATG1220.3200167877659156No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGCGGAGCAAGTCAGAA1210.31739369934160483No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGTACGGCAAGTCTGAT1180.30952443406867247No Hit
TCCGGAATTATTGGGCGTAAAGAGGGAGCAGGCGGCACTAAGGGTCTGTG1180.30952443406867247No Hit
TCCGGAATTACTGGGTGTAAAGGGTGCGTAGGTGGTATGGCAAGTCAGAA1130.29640899194711856No Hit
TCCGGATTTACTGGGTGTAAAGGGCGCGCAGGCGGGCCGGCAAGTTGGAA1060.27804737297694304No Hit
TCCGGATTTACTGGGCGTAAAGGGAGCGTAGGCGGACTTTTAAGTGAGAT1010.26493193085538913No Hit
ATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTCTGTAAGACAGAT930.24394722346090286No Hit
ATCGGAATCACTGGGCGTAAAGCGCACGTAGGCTGTTATGTAAGTCAGGG870.22820869291503817No Hit
TCCGGAATTACTGGGTGTAAAGGGAGCGTAGGCGGGATCGCAAGTCAGAT860.22558560449072737No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGGCGGGAGAACAAGTTGGAA860.22558560449072737No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCATTGCAAGCCAGAT850.2229625160664166No Hit
TCCGGAATCATTGGGCGTAAAGCGCGCGCAGGCGGGCTTTCAAGCGGCGG830.21771633921779504No Hit
TCCGGAATCATTGGGCGTAAAGGGTGCGTAGGTGGCGTACTAAGTCTGTA720.18886236655037641No Hit
TCCGGATTTACTGGGCGTAAAGGGTGCGTAGGCGGTCTTTTAAGTCAGGA700.18361618970175486No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGTGGACATGTAAGTCAGTT690.18099310127744406No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGCCCGGCAGGCCGGGG690.18099310127744406No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGTAAAGCAAGTCTGAA640.16787765915589015No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGCAGACGGCGATGCAAGTCTGGA630.16525457073157937No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGACGCAAGTCTGAA610.1600083938829578No Hit
TCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGTTCCATAAGTCTGAT600.157385305458647No Hit
TCCGGATTTACTGGGTGTAAAGGGAGTGTAGGTGGCCAGGCAAGTCAGAA580.15213912861002543No Hit
TCCGGATTTACTGGGTGTAAAGGGCGTGTAGGCGGGAGTGCAAGTCAGAT570.14951604018571465No Hit
TCCGGATTTACTGGGTGTAAAGGGTGCGTAGGCGGATTGGCAAGTCAGAA570.14951604018571465No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGCAGCAAGTCTGAT570.14951604018571465No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCAAGGCAAGTCTGAT570.14951604018571465No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATGGGCAAGTCTGAT560.14689295176140388No Hit
TCCGGATTTACTGGGTGTAAGGGGTGCGTAGGCGGATTGGCAAGTCAGAA550.14426986333709307No Hit
CTCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGAT530.13902368648847152No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGACTGGCAAGTCTGAT530.13902368648847152No Hit
TCCGGATTTATTGGGCGTAAAGCGAGTGCAGGCGGGTCAATAAGTCTGAT490.1285313327912284No Hit
TCCGGATTCATTGGGCGTAAAGCGCGCGTAGGCGGAGCGCTAAGCGGGAC480.1259082443669176No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCGGGGTATTAAGTCAGTT470.12328515594260683No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGATTAGCAAGTCTGAT450.11803897909398527No Hit
TCCGGATTTATTGGGTTTAAAGGGAGCGTAGGCCGGAGGTTAAGCGTGTT450.11803897909398527No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGAATGGCAAGTCTGAT450.11803897909398527No Hit
TCCGGAATGATTGGGCGTAAAGGGTGCGTAGGTGGCAGATCAAGTCTGGA400.10492353697243134No Hit
TCCGGATTTATTGGGCGTAAAGGGCTCGTAGGCGGCTCGTCGCGTCCGGT400.10492353697243134No Hit
TCCGGATTTACTGGGTGTAAAGGGAGCGTAGACGGCGATGCAAGTCTGAA390.10230044854812055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)