FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005499147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005499147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36302
Sequences flagged as poor quality0
Sequence length200
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGT506013.93862597102088No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGA366810.104126494408023No Hit
TTCGAGCCTCAGCGTCAGTTATCGTCCAGTAAGCCGCCTTCGCCACTGGT34999.638587405652581No Hit
TTCGAGCCTCAACGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT22946.319211062751363No Hit
TTCGAGCCTCAACGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT21645.961104071401025No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCAAGCCGCCTTCGCCACTGGT14083.878574183240593No Hit
TTCGAGCATCAATGTCAGTTACAGGTTAGTAAGCTGCCTTCGCAATCGGA9612.647237066828274No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGAT8902.4516555561677045No Hit
TTCGTGCATGAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT7602.0935485648173655No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCACTGGT6431.7712522726020605No Hit
TTCGAGCCTCAACGTCAGTGGCGGTTTAGCAGGCTGCCTTCGCAATCGGG6281.72993223513856No Hit
TTCGAGCCTCAGCGTCAGTTACCGTCCAGTAAGCCGCCTTCGCCACTGGT5701.570161423613024No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGAT5201.4324279654013552No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGCAGGCTGCCTTCGCAATCGGA5181.4269186270728884No Hit
TTCGAGCATCAGCGTCAGTTACAATCCAGTAAGCTGCCTTCGCAATCGGA5131.4131452812517216No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACTGGT4441.2230731089196187No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT3991.0991129965291169No Hit
TTCGAGCCTCAGCGTCAGTTGTCGTCCAGTAAGCCGCCTTCGCCACTGGT3450.9503608616605146No Hit
TTCGCTCCTCAGCGTCAGTAACGGCCCAGAGACCTGCCTTCGCCATTGGT3070.8456834334196464No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTGAGCCGCCTTCGCCTCCGGT3040.8374194259269462No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGCAAGCCGCCTTCGCCACTGGT3020.8319100875984795No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGACCGCCTTCGCCACTGGT2890.7960993884634455No Hit
TTCGAGCATCAGTGTCAGTGACAGTCTAGTGAGCTGCCTTCGCAATCGGA2880.7933447192992122No Hit
TTCGTGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT2640.7272326593576112No Hit
TTCGAGCCTCAACGTCAGTCGCGGCTTAGCAGGCTGCCTTCGCAATCGGG2470.6804032835656438No Hit
TTCGTGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT2240.6170458927882762No Hit
TTCGAGCCTCAACGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT2150.5922538703101757No Hit
TTCGTGCCTCAGCGTCAGTTGAGCGCCGGCAGGCTGCCTTCGCAATCGGG2120.5839898628174757No Hit
TTCGTGCCTCAACGTCAGATATAGTTTGGTAAGCTGCCTTCGCAATCGGT1920.5288964795328082No Hit
TTCGAGCATCAGTGTCAGTTGCAGTCCAGTGAGCTGCCTTCGCAATCGGA1890.520632472040108No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGTAAGCCGCCTTCGCCACTGGT1880.5178778028758746No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGAAAGCCGCCTTCGCCACTGGT1840.506859126218941No Hit
CTCGCGCATCAGCGTCAGAACGAGCCTGGGAAGCTGCCTTCGCTATCGGG1800.49584044956200757No Hit
TTCGAGCATCAGCGTCAGTTACAGTCCAGTAAGCTGCCTTCGCAATCGGA1680.46278441959120714No Hit
TTCGAGCCTCAGCGTCAGTAAAGGCCCAGTAAGCCGCCTTCGCCACCGAT1670.4600297504269737No Hit
TTCGAGCCTCAGCGTCAGTCATCGTCCAGTAAGCCGCCTTCGCCACTGGT1650.45452041209850697No Hit
TTCGGGACTGAGCGTCAGTTATGCGCCAGATCGTCGCCTTCGCCACTGGT1470.40493636714230624No Hit
TTCGAGCCTCAACGTCAGTTGCCGTCCAGTAAGCCGCCTTCGCCACTGGT1340.3691256680072723No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAAGCCGCCTTCGCCACTGGT1330.3663709988430389No Hit
TTCGTGCATCAGCGTCAGTCATGGCTTGGCAGGCTGCCTTCGCAATCGGG1330.3663709988430389No Hit
TTCGTGCCTCAGCGTCTGTTTCACCCTAGCAGGCTGCCTTCGCAATCGGG1320.3636163296788056No Hit
TTCGAGCCTCAACGTCAGTGGCGGTTTAGCAGGCTGCCTTCGCCATCGGG1290.35535232218610546No Hit
TTCGCGCCTCAGCGTCAGTTAATGTCCAGCAGGCCGCCTTCGCCACTGGT1250.34433364552917195No Hit
TTCGAGCCTCAACGTCAGTGGCGGTTTAGCAGGCTGCTTTCGCAATCGGG1120.3085229463941381No Hit
TTCGTGCCTCAGTGTCAGTAACAGTCCAGCAGGCCGCCTTCGCCACTGGT1030.2837309239160377No Hit
TTCGAGCCTCAGCGTCAGTGCTGCCCCGGCACAATGCCTTCGCGATCGGA980.2699575780948708No Hit
TTCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGTCGCCTTCGCCACTGGT960.2644482397664041No Hit
TTCGAGCCTCAGCGTCATTGTCAGTCCAGTAAGCCGCCTTCGCCACTGGT950.2616935706021707No Hit
TTCGCGCCTCAGCGTCAGTGGCGGCCCAGAAGGCTGCCTTCGCCATCGGT880.24241088645253706No Hit
TTCGAGCCTCAGCGTCAGTAATCGTCCAGTAAGCCGCCTTCGCCACCGGT850.2341468789598369No Hit
TTCGCGCCTCAGCGTCAGTTTCGGCCCAGAGAGCTGCCTTCGCCGTCGGT810.22312820230290342No Hit
TTCGCGCCTCACCGTCAGTTATCGTCCAGCAATCCGCCTTCGCCACTGGT810.22312820230290342No Hit
TTCGTGCTTCAGTGTCAGTTATGGTTTAGTAAGCTGCCTTCGCAATCGGA810.22312820230290342No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGTAAGCCGCCTTCGCCACTGGT780.21486419481020327No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGTAAGCCGCCTTCGCCACTGGT750.20660018731750318No Hit
TTCGAGCCTCAGCGTCAGTAGTCGTCCAGTAAGCCGCCTTCGCCACTGGT630.17354415734670267No Hit
TTCGTGCCTCAGTGTCAGTTTCAGTCCAGTAAGCCGCCTTCGCCACTGGT620.17078948818246928No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAGGCCGCCTTCGCCACTGGT580.15977081152553577No Hit
TTCGCGCCTCAGCGTCAGTTGTCGTCCAGCAAGCCGCCTTCGCCACTGGT580.15977081152553577No Hit
TTCGAGCATCAGCGTCAGTTACACTCCAGTGAGCTGCCTTCGCAATCGGA560.15426147319706904No Hit
TTCGAGCCTCAGCGTCAGTTAAAGCCCAGCAGGCCGCCTTCGCCACTGGT550.15150680403283567No Hit
TTCGAGCCTCAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT550.15150680403283567No Hit
TTCGTGCATTAGCGTCAGTCATGTCCCAGAGGGCTGCCTTCGCCATCGGT550.15150680403283567No Hit
TTCGAGCCTGAACGTCAGTTACTGTCCAGCAAGCCGCCTTCGCCACTGGT500.13773345821166877No Hit
TTCGAGCCTCAATGTCAGTTGCAGCTTAGCAGGCTGCCTTCGCAATCGGG500.13773345821166877No Hit
TTCGAGCCTCAGTGTCAGTTACAGTCCAGCGAGCCGCCTTCGCCTCCGGT500.13773345821166877No Hit
TCGAGCCTCAGCGTCAGTTGGTGCCCAGTAGGCCGCCTTCGCCACTGGTG470.12946945071896865No Hit
TTCGAGCCTCAACGTCAGTGGCGGTTTTGCAGGCTGCCTTCGCAATCGGG470.12946945071896865No Hit
TTCGCGCCTCAGCGTCAGTTTCAGTCCAGAAAGCCGCCTTCGCCACTGGT440.12120544322626853No Hit
TTCGTGCCTCAGCGTCAGTTACAGTCCAGAGAGCCGCCTTCGCTACTGGT430.11845077406203515No Hit
TTCGCGCCTCAGCGTCAGTTATCGTCCAGTTGACCGCCTTCGCCACTGGT420.11569610489780179No Hit
TTCGCATCTCAGCGTCAGACACAGTCCAGAAAGGCGCCTTCGCCACTGGT410.11294143573356841No Hit
TTCGCGCCTCAGCGTCAGTTACAGTCCAGTTAGTCGCCTTCGCCACTGGT400.11018676656933502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)